毛头鬼伞菌盖中蛋白质提取条件优化及氨基酸分析
Optimization of Protein Extraction from Pileus of Coprinus comatus and Analysis of Its Amino Acid
苏玉春 1俞佳宝 1李文君 2汪树生 1向应欣1
作者信息
- 1. 吉林农业大学生命科学学院,长春 130118;秸秆生物学与利用教育部重点实验室,长春 130118
- 2. 长春国际卫生旅行保健中心,长春 130062
- 折叠
摘要
采用超声辅助氢氧化钠碱法提取毛头鬼伞菌盖蛋白质,并通过单因素试验和正交试验,分析料液比、温度、时间、超声功率、pH对蛋白质提取率的影响,确定了最佳提取工艺:提取液pH 11,提取温度50℃,料液比为1∶40,提取时间60 min,超声功率为200 W,在此条件下提取率为33.18%.采用柱前衍生高效液相色谱法分析氨基酸的组成,并通过氨基酸比值系数分(SRC)、化学评分(CS)等指标对菌盖蛋白质进行初步营养价值评价.菌盖中氨基酸种类齐全,人体必需氨基酸占总氨基酸的35.9%,EAA/NEAA为0.58,符合WHO提出的推荐值.
Abstract
Ultrasonic assisted sodium hydroxide alkaline method was used to extract protein from pi-leus of Coprinus comatus.Through single factor test and orthogonal test,the effects of solid-liquid ra-tio,temperature,time,ultrasonic power,and pH value on protein extraction rate were analyzed,and the optimal extraction process was determined.The composition of amino acids was analyzed by pre-column derivatized high performance liquid chromatography,and the nutritional value of amino acids was preliminarily evaluated by amino acid ratio coefficient score(SRC)and chemical score(CS).The optimal extraction conditions were as follows:pH 11,50℃,solid-liquid ratio 1∶40,60 min,and ul-trasonic power 200 W.Under these conditions,the extraction rate was 33.18%.There are a variety of amino acids in the pileus of Coprinus comatus.The essential amino acids accounted for 35.9%of the total amino acids,and EAA/NEAA was 0.58,which is close to the WHO recommended value.
关键词
毛头鬼伞/菌盖/蛋白质提取/氨基酸Key words
Coprinus comatus/pileus/protein extraction/amino acid引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据