3种除草剂在玉米中的残留分析方法及样品储藏稳定性
Residue Analysis Method and Sample Storage Stability of Three Her-bicides in Maize
冷金蔓 1张晟 1孙晓伟 1高玉雪 1侯志广 1逯忠斌1
作者信息
- 1. 吉林农业大学植物保护学院,长春 130118
- 折叠
摘要
建立高效液相色谱-串联质谱法检测乙草胺、苯唑草酮和氯氟吡氧乙酸在玉米中的残留量及储藏稳定性.样品经含0.3%甲酸的乙腈提取,以乙腈-0.1%甲酸+4.5 mmol/L甲酸铵+0.5 mmol/L氟化铵混合流动相进行梯度洗脱,再进行高效液相色谱串联质谱分析.结果表明:在0.001～0.5 mg/L,3种除草剂线性良好(R≥0.998 3),平均添加回收率76.7%～102.2%,相对标准偏差1.2%～10.5%.在-10℃条件下,乙草胺、苯唑草酮和氯氟吡氧乙酸在玉米残留样品中至少可以稳定储藏30 d;在-20℃条件下至少可以稳定储藏210 d.检测方法为乙草胺、苯唑草酮和氯氟吡氧乙酸的残留检测与储藏稳定性分析提供参考.
Abstract
A method for the determination of acetochlor,topramezone and fluroxypyr in maize by HPLC MS was established.The samples were extracted with acetonitrile containing 0.3%formic acid,and the mobile phase consisted of acetonitrile-0.1%formic acid+4.5 mmol/L ammonium formate+0.5 mmol/L ammonium fluoride for gradient elution.The results showed that in the range of 0.001-0.5 mg/L,the three herbicides showed good linearity(R≥0.998 3),the average re-covery rate of the three pesticides was 76.7%-102.2%,and the relative standard deviation was 1.2%-10.5%.Under-10℃comditions,acetochlor,topramezone and fluroxypyr could be stored stably in maize residue samples for at least 30 d.Under-20℃conditions,acetochlor,toprame-zone and fluroxypyr could be stored in maize residue samples for at least 210 d.This method pro-vides reference for residual detection and storage stability analysis of acetochlor,topramezone and fluroxypyr.
关键词
乙草胺/苯唑草酮/氯氟吡氧乙酸/玉米/残留分析/储藏稳定性Key words
acetochlor/topramezone/fluroxypyr/maize/residue analysis/storage stability引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据