Active Ingredient Tracking of Sika Deer Pilose Anlter Extracts Pro-moting 1-methyl-4-phenylpyridine Ion Induced Neuronal Damage Repairation
The sika deer pilose anlter extracts(PAEs)were divided into different components by dif-ferent methods.The PC12 cell injury model induced by 1-methyl-4-phenylpyridine ion(MPP+)was screened to determine the neuroprotective effects of PAEs.The active components lay the foundation for the next step of new drug development.PAEs were extracted by a patented method in our labora-tory,and treated separately by ultrafiltration centrifugation and heat treatment to obtain different components of PAEs,PAEsH(heat treatment),and PAEs30(ultrafiltration centrifugal molecular weight＞30 kDa components).Their protective effects on MPP+-induced PC12 cell damage were ob-served,and separation was further tracked.The most active component PAEs30 was selected for fur-ther heat treatment and enzymatic hydrolysis to obtain PAEs30-H(heat treatment)and PAEs30-E(enzy-matic hydrolysis).Their protective effects on MPP+-induced PC12 cell damage were observed,and tracking of separation was further carried out to determine the effective active component.Compared with the MPP+injured PC12 cell model,PAEs,and PAEs30 at concentrations above 0.5 mg/mL could significantly inhibit MPP+damage of PC12 cells,with PAEs30 component having the strongest activ-ity.After heat treatment and enzymolysis treatment of PAEs30(PAEs30-H and PAEs30-E),each compo-nent had a significant neuroprotective effect on MPP+injured PC12 cells,and concentrations of 0.125 mg/mL and above could significantly improve cell survival rate.PAEs repaired the damage of MPP+-induced nerve cell.The main active components were initially identified as protein compo-nents with thermal stability and molecular weight of 10 kDa or 35 kDa.The research provides experi-mental data for subsequent quality standard formulation and extraction process.
pilose anlter extractactivity tracking separationrepairation of neuronal damageultrafiltration centrifugation
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