Isolation,Identification and Genome-wide Analysis of Clostridium bu-tyricum from Pantholops hodgsonii
In this study,multiple bacterial strains were isolated from the feces of Pantholops hodg-soni by anaerobic microbial isolation and culture technology.A strain of Clostridium butyricum FC36 was identified by morphological observation,physiological and biochemical tests,and 16S rRNA se-quencing technology.In order to explore the potential probiotic function of isolated strain,its whole genome sequence was determined.Assembly and functional analysis showed that the genome size was 4.4 Mbp,G+C content was 28.53%,and contained 4 022 protein-coding genes.A total of 532 carbohydrate-enzyme family genes,1 070 metabolic pathway related genes,3 244 homologous pro-tein cluster genes,and 2 bacteriocin coding genes were annotated.At the same time,in vitro experi-ments were carried out to study its probiotic function,including growth curve and pH change curve measurement,amylase production and short-chain fatty acid detection,tolerance evaluation of bacte-riostatic test.The results showed that Clostridium butyricum FC36 had a fast growth rate,could pro-duce amylase and a variety of short-chain fatty acids,had strong tolerance,and had certain inhibi-tory ability against Escherichia coli and Salmonella.In summary,a strain of Clostridium butyricum with excellent performance was isolated in this study,which laid a foundation for the development of relevant probiotics in clinical practice.
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