Using Bacillus natto and Bletilla striata as experimental materials,the effects of Bacillus natto on the main quality and active metabolites of Bletilla striata were studied by physicochemical detection and metabolomics techniques.The results showed that Bacillus natto could promote the accumulation of starch,protein and polysaccharide,increase the contents of 4 amino acids and 3 trace elements in Bletilla striata.Bacillus natto could promote the accumulation of 6 bibenzyl compounds,4 dihydrophenanthrene and phenanthrene compounds,8 flavonoids active substances.Bacillus natto can relieve heavy-metal pollution and degrade pesticide residues.