Study on Optimization of Collagen Extraction Technology of Deer Sinews
Sika deer sinews were used as raw materials,single factor test and orthogonal test were used to optimize the collagen extraction process of deer sinews.The optimal process conditions were A1B2C2D2,acetic acid concentration was 0.50 mol/L,solid to liquid ratio was 1∶40 g/mL,soaking time was 30 hours,and the ratio of trypsin to substrate was 1∶700.Under this process condition,the recovery rate of collagen in deer sinews could reach 59%.