正交试验优化黄芪多糖的提取工艺研究
Study on Optimization of Extraction Process of Astragalus Polysaccharides by Orthogonal Test
王雪 1闫峰1
作者信息
- 1. 吉林农业科技学院生物与制药工程学院,吉林 132101
- 折叠
摘要
以黄芪为试材,利用水提醇沉法提取多糖,采用正交试验考查乙醇浓度、提取时间、提取温度、料液比4个试验因素,以黄芪多糖提取率为试验指标进行工艺条件优化.结果表明:黄芪多糖提取工艺为提取时间1.5 h、提取温度100℃、料液比1∶6g/mL、90％的乙醇隔夜沉淀.在此条件下,黄芪多糖提取率最高,为2.85％.通过正交试验设计优化黄芪多糖提取工艺稳定可行,为黄芪多糖提取方法的研究提供参考.
Abstract
Astragalus was used as test material.Polysaccharides were extracted by water boiling and alcohol precipitation method.Orthogonal test was used to investigate the factors of ethanol concentration,extraction time,extraction temperature,and the ratio of material to liquid.The extraction rate of Astragalus polysaccharide was used as the test index to optimize the process conditions.The results showed that the extraction process of Astragalus polysaccharide was the extraction time 1.5 hours,the extraction temperature 100 ℃,the ratio of material to liquid 1∶6 g/mL,and 90％alcohol was precipitated overnight.Under this condition,the extraction rate of Astragalus polysaccharide was the highest,which was 2.85％.It is stable and feasible to optimize the extraction process of Astragalus polysaccharide by orthogonal test design,which provides a reference for the study of Astragalus polysaccharide extraction method.
关键词
黄芪/多糖/提取/水提醇沉法Key words
Astragalus/polysaccharide/extraction/water boiling and alcohol precipitation method引用本文复制引用
基金项目
吉林农业科技学院大学生科技创新创业项目(吉农院合字[2021]第077号)
吉林省科技厅项目(20220202013NC)
出版年
2024
NETL
NSTL
万方数据