With the advent of next-generation sequencing technology,gene sequence has been widely studied.Due to the complexity of genetic material,technical errors introduced during sample preparation,sequencing,and analysis,and systematic biases between different sequencing platforms,high-throughput sequencing results suffer in terms of accuracy and consistency across platforms.However,the use of standard materials in the sequencing process can solve these problems.Next-generation sequencing reference materials are usually genetic materials with good characteristics or synthetic reference materials added and simulated electronic data sets.The application of next-generation sequencing reference materials will help calibrate the measurement results of next-generation sequencing and evaluate the instrument performance,which is crucial to ensure the accuracy and consistency of sequencing results.
biometricsnext-generation sequencingreference materialquality control
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