In the traditional hydrostatic excitation method for hydrophone calibration system,the influence of inertial force on equivalent sound pressure is inconsistent due to the lack of constraint of the connecting hose during water vibration in the connecting hose,resulting in a large uncertainty introduced by measurement repeatability,which affects the uncertainty of hydrophone sensitivity calibration.A hydrostatic excitation method for hydrophone calibration based on motion trajectory constraint is proposed to solve this problem.This method designs a motion constraint device to control the same motion trajectory of the communicating hose during vibration,establishes a mathematical model based on this,determines the impact of the vibration of the communicating hose on the equivalent sound pressure,and derives an equivalent height calculation formula that is not limited by the open-circuit voltage measurement data of the hydrophone.A hydrostatic excitation method hydrophone calibration device based on motion trajectory constraints is built and compared with traditional methods.Experimental results indicate that,compared to the traditional hydrostatic pressure stimulation method,the proposed method reduced the uncertainty in hydrophone sensitivity calibration from 0.34 dB(k=2)to 0.25 dB(k=2),with the uncertainty introduced by measurement repeatability decreasing from 0.12 dB to 0.02 dB.