Effects of breast milk microbiota on intestinal microbiota of 6-month-old infants
Objective:To investigate the effect of breast milk with bacteria on intestinal microbiota of 6-month-old infants based on 16S rRNA sequencing technology.Methods:A total of 12 postpartum women and their infants were included in this study.The 6-month-old postpartum milk and 6-month-old infant fecal samples were collected.According to the 6-month-old postpartum breast milk with or without bacteria,the breast milk was divided into sterile breast milk group and bacterial breast milk group,and the diversity and species difference of intestinal microbiota of 6-month-old infants fed by sterile breast milk and bacterial breast milk were compared.Results:In 6-month-old postpartum breast milk samples of 12 cases,microbiota was found in 7 cases,and the detection rate of microbiota in breast milk samples was 58.33%.There was no significant difference in α and β diversity between 6-month-old infants fed with bacterial breast milk and 6-month-old infants fed by sterile breast milk.The relative abundance of Clostridium_innocuum,unidentified_Erysipelotrichaceae,Erysipelotrichia,Erysipelotrichales and Erysipelotrichaceae in intestinal microbiota of 6-month-old infants fed by bacterial breast milk was significantly higher than that of 6-month-old infants fed by sterile breast milk(P<0.05).The relative abundance of Lactobacillaceae and Lactobacillus in intestinal microbiota of infants fed by sterile breast milk was significantly higher than that of infants fed with by bacterial breast milk(P<0.05).Conclusion:The 6-month-old postpartum breast milk may without bacteria.The presence or absence of microbiota in breast milk may not change the diversity of intestinal microbiota of 6-month-old infants,but it can change the structure of intestinal microbiota of 6-month-old infants.
breast milkbreast milk microbiotabreastfeedinginfantintestinal microbiota
NETL
NSTL
万方数据
