首页|630nm LED照射对小鼠巨噬细胞向M1型极化的抑制作用

630nm LED照射对小鼠巨噬细胞向M1型极化的抑制作用

扫码查看
目的:探讨630 nm发光二极管(LED)照射对体内外小鼠巨噬细胞向M1 型极化及功能的影响.方法:以小鼠巨噬细胞系RAW264.7 细胞和原代腹腔巨噬细胞为实验对象,在脂多糖(LPS)和干扰素-γ(IFN-γ)诱导其向M1 型极化前、后用光照功率密度为8 mW/cm2 的630 nm LED照射细胞,利用CCK-8 法测定细胞存活率,流式细胞术检测细胞表面活化/共刺激分子CD80 和CD86 的表达水平,实时荧光定量PCR(RT-qPCR)和/或酶联免疫吸附分析(ELISA)检测肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)等的表达与分泌.结果:630 nm LED 照射对 RAW264.7 细胞的存活率无显著影响,但可显著降低经 LPS 和 IFN-γ 极化后RAW264.7 细胞的CD80、CD86 蛋白表达水平及促炎细胞因子TNF-α、IL-6 和IL-1β的mRNA表达量(P<0.05).体内外630 nm LED照射可抑制在体外经LPS和IFN-γ刺激极化后小鼠原代腹腔巨噬细胞TNF-α、IL-6 的分泌水平(P<0.05).结论:630 nm LED照射可抑制巨噬细胞向M1 型极化和促炎因子的表达与分泌,这可为M1 型巨噬细胞参与的炎症性疾病的治疗提供一些新的思路.
Inhibitory effect of 630 nm LED irradiation on the polarization of murine M1-type macrophages
Objective:To investigate the effect of 630 nm LED irradiation on the polarization and function of murine M1-macrophages both in vitro and in vivo.Methods:Murine macrophage cell line RAW264.7 and primary peritoneal macrophages were irradiated with 630 nm LED(at a power density of 8 mW/cm2)either before or after the induction of M1-polarization by lipopolysaccharide(LPS)and interferon-γ(IFN-γ).Cell viabilities were measured by CCK-8 assay.Levels of surface activation/costimulatory molecules CD80&CD86 were determined by flow cytometry,and expressions of proinflammatory cytokines including tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)&interleukin-1β(IL-1β)were analyzed by quantitative real-time PCR(RT-qPCR)and/or enzyme-linked immunosorbent assay(ELISA).Results:Despite no effect on cell viabilities,630 nm LED irradiation,either before or after the induction of M1-polarization by LPS and IFN-γ,not only significantly reduced surface expressions of CD80 and CD86 on RAW264.7 cells,but also hampered their mRNA exprssion and productions of pro-inflammatory cytokines TNF-α,IL-6 and IL-1β(P<0.05).Moreover,pretreating mice with 630 nm LED followed by in vitro LED irradiation of cells repressed the ability of primary murine peritoneal macrophages to secrete TNF-α and IL-6 upon activation by LPS and IFN-γ in vitro as well(P<0.05).Conclusion:630 nm LED irradiation blunts the M1 polarization and pro-inflammatory cytokine production of murine macrophages,which may provide new therapeutic strategy for the clinical treatment of M1 macrophage-mediated inflammatory diseases.

630 nm LEDmacrophage polarizationM1 macrophageinflammatory responses

王洪民、郑维龙、赵赛、何军、易斌、高晓明、王俊

展开 >

苏州大学 生物医学研究院,江苏 苏州 215123

北京创盈光医疗科技有限公司,北京 100176

630nm LED 巨噬细胞极化 M1型巨噬细胞 炎症反应

国家重点研发计划项目

2017YFB0403805

2024

暨南大学学报(自然科学与医学版)
暨南大学

暨南大学学报(自然科学与医学版)

CSTPCD北大核心
影响因子:0.996
ISSN:1000-9965
年,卷(期):2024.45(3)
  • 5