Prediction of community diversity and ecological functions of endophytic fungi and rhizosphere fungi in Lilium lancifolium ker gawl
Objective:The community structure of endophytic fungi in the scale leaves and roots of the lilium lancifolium,as well as the fungi in the rhizosphere soil,was analyzed.Furthermore,the ecological functions of endophytic fungi in the scale leaves,a medicinal part,were predicted.This approach aimed to identify functional groups closely associated with the active ingredients of L.lancifolium.Methods:The Illumina MiSeq sequencing technology was utilized to sequence the ribosomal DNA(rDNA)of the internal transcribed spacer(ITS)region within the rhizosphere and endophytic fungi of L.lancifolium.The operational taxonomic units(OTUs)were used to functionally classify the scale leaves endophytic fungal community in FUNGuild software.Results:① Ascomycota and Basidiomycetes are the predominant phyla in L.lancifolium.Cutaneotrichosporon and Fusarium are the dominant genera found on scale leaves.Cutaneotrichosporon is significantly more prevalent in scale leaves than in roots and rhizosphere soil.②The scale leaves of L.lancifolium are mainly divided into eight functional groups:undefined saprophytes,plant pathogens,plant endophytes-plant pathogens,animal pathogens-undefined saprophytes,ectomycorrhizal fungi,litter saprotroph-plant pathogen,endophyte-undefined saprotroph and wood saprotroph.Undefined saprotroph is the most dominant group of scale leaves,and OTUs mainly belong to Nemania.Conclusion:Cutaneotrichosporon and Fusarium may be closely related to the active ingredient of L.lancifolium.
endophytic fungicommunity diversity analysisecological function predictionhigh-throughput sequencing
万方数据
维普
