The effect of platycladus orientalis leaf extract on blood coagulation and its mechanisms
Objective To investigate the effect of Platycladus orientalis leaves on blood coagulation and its related mechanisms.Methods In vitro coagulation experiment:prepared the extract of Platycladus orientalis leaves by petroleum ether,ethyl acetate,n-butanol and water.Took the blood from the common carotid artery of rabbits,and ob-served the effect of the extract of Platycladus orientalis leaves by water,petroleum ether,n-butanol and ethyl acetate on blood coagulation with the clotting plate method.Using RT,APTT,PT,and TT as indicators,observed the effects of Platy-cladus orientalis leaves and its extracts on blood coagulation in vitro experiments.In vivo coagulation experiment:Healthy Kunming mice were randomly divided into low and high concentration groups of water extract from Platycladus orientalis leaves.Low and high concentration groups of ethyl acetate extract.Low and high concentration groups of n-butanol ex-tract,as well as blank group.Using coagulation time,APTT,PT,TT,and coagulation factor ⅩⅢ as indicators,the effects and related mechanisms of Platycladus orientalis leaves and its extracts on blood coagulation were studied through in vivo experiments.The effect of extract of Platycladus orientalis leaves on gastric ulcer bleeding model:A gastric ulcer bleed-ing model was prepared by gavage with 5ml/kg anhydrous ethanol.The effect of Platycladus orientalis leaves and its ex-tract on gastric bleeding model was observed using gastric bleeding area and gastric mucosal cell morphology after gastric tissue sectioning as indicators.Results In vitro experiment:Compared with the blank control group,petroleum ether,ethyl acetate,n-butanol extract,and water extract significantly prolonged coagulation time and RT(P<0.05);The n-bu-tanol extract,water extract significantly prolonged APTT and PT(P<0.05),and ethyl acetate extract significantly pro-longed PT(P<0.05).In vivo experiment:Compared with the blank control group,the low concentration group of ethyl acetate,n-butanol and water extract significantly shortened the coagulation time(P<0.05);The high concentration group of ethyl acetate extract and the low concentration group of n-butanol extract significantly shortened APTT and PT separately(P<0.05),while the high concentration group of n-butanol and water extract significantly prolonged TT(P<0.05);Compared with the blank control group,the low concentration group of ethyl acetate,the high and low concentra-tion group of n-butanol extract showed a significant increase in plasma factor ⅩⅢ content(P<0.05).Compared with the model control group,the areas of gastric bleeding in the high and low concentration groups of ethyl acetate extract,high and low concentration groups of n-butanol extract,and high and low concentration groups of water extract were significant-ly reduced(P<0.05).The low concentration group of ethyl acetate extract had the smallest area of gastric bleeding.Conclusion In vivo experiments,Platycladus orientalis leaves mainly promote coagulation,with ethyl acetate as the main hemostatic site,and low concentrations of ethyl acetate extract have better hemostatic effects.
Platycladus orientalis leavesBlood coagulationGastric bleeding
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