Rapid determination of antioxidant activity of Guizhi formula granules based on near infrared spectroscopy
Objective To establish a near infrared spectroscopy method for the rapid determination of antioxidant activity of Guizhi formula granules.Methods A portable near-infrared spectrometer was used to collect diffuse near-infrared spectral data of 30 batches of samples of Guizhe formula particles.The original spectra were preprocessed by different spectral pretreatment methods.PLS and support vector regression(SVR)were used to optimize the wave number interval,and DPPH,ABTS and FRAP were used as reference methods to establish a quantitative analysis model of antioxidant activity of Guizhi formula granules.Results The near-infrared spectroscopy method can effectively pre-dict the antioxidant activity of different batches of Guizhi formula particles.The predictive correlation coefficient(Rp)and corrected correlation coefficient(Rc)of DPPH method for PLS model were 0.769 and 0.795 respectively.The Rp value and Rc value of the SVR model corresponding to ABTS method are 0.928 and 0.992.The Rp value and Rc value of the SVR model corresponding to FARp method are 0.573 and 0.926.Conclusion The near infrared spectroscopy can be used to predict the antioxidant activity of different batches of Guizhe formula granules,and has certain reference significance for the rapid evaluation of biological activity of other Chinese medicine formula granules.
Near infrared spectroscopyGuizhi formula granuleAntioxidant activity
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