Objective To evaluate the expression and clinical relevance of zinc finger protein GLIS3 in hepatocel-lular carcinoma(HCC).Methods Expression data for GLIS3 were extracted from TCGA,GTEx,and GEO databases for analysis involving 2101 HCC and 1448 normal liver samples.GLIS3 mRNA expression was quantified in HCC cell lines(MHCC-97H,SK-HEP-1)and a normal liver cell line(LX2)using real-time quantitative PCR.GLIS3 protein expression levels were assessed via immunohistochemistry in 96 paired samples of HCC and adjacent non-tumor tissue.Results The results of database analysis showed that GLIS3 expression was significantly lower than that in HCC tissues compared to normal liver tissue(P<0.001).Lower GLIS3 expression was confirmed in MHCC-97H and SK-HEP-1 HCC cell lines relative to the normal liver cell line(t=2.933,P<0.05;t=11.892,P<0.05).There is statistically significant difference between GLIS3 protein expression and tumor number in HCC patients.GLIS3 was associated with prognosis in HCC patients.Conclusion Reduced GLIS3 expression in HCC suggests its potential as a therapeutic target and prognostic biomarker.
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