Effects of neonatal repeated inhalation of sevoflurane on hippocampal volume and Tau in cerebrospinal fluid of adult rats
Objective To investigate changes of hippocampal volume and Tau in cerebrospinal fluid(CSF)of adult rats with neonatal repeated inhalation of sevoflurane,and to confirm magnetic resonance imaging(MRI)and CSF as the basis for inferring the effects of sevoflurane on cognitive function.Methods 32 neonatal SD rats were randomly divided into the experimental group(n=16)and the control group(n=16).Rats in experimental group inhalated 2.6%sevoflurane for 2 hours on postnatal 7th day(P7),P14 and P21 and rats in control group inhalated 1L/minO2+1L/min Air for 2 hours on the same days.After being naturally fed until adulthood,the rats underwent cranial MRI scanning on postnatal day 96(P96)to measure relevant indicators such as hippocampal volume.Immediately after the MRI scan-ning,cerebrospinal fluid(CSF)was collected.Following the completion of sample collection,the OD450mm values of the samples were measured according to the instructions of the ELISA kit.Finally,the Tau concentration was calculated based on the OD450mm values.Results Compared with control group,the brain volume(BV),left hippocampal volume(LHV),right hippocampal volume(RHV),brain length(BL)in experimental group were significantly lower(P<0.05)BV,LHV,RHV,and BL in control group were 2.19±0.10cm3,45.85±4.60mm3,46.04±2.90mm3,15.38±0.51mm,respectively;BV,LHV,RHV,and BL in experimental group were 1.88±0.10cm3,41.54±4.59mm3,43.12±2.70mm3,14.70±0.48mm,respectively,the differences were statvstically significant(t=-6.20,-1.88,-2.16,-2.83,P<0.05).The concentration of Tau protein in cerebrospinal fluid(CSF)was lower in the experimental group[control group:(42.62±12.50)ng/mL;experimental group:(28.93±6.13)ng/mL],with a statistical difference(t=-2.23,P<0.05).When comparing the other indicators between the groups,no statistically significant differences were found.Conclusions With neonatal repeated inhalation of sevoflurane,the total content of Tau in CSF of adult rats decreased,however,it can not be considered that the abnormal phosphorylated Tau decreased.The hippocampal volume decreased in adult,which can be considered that the MRI measurement of hippocampal volume has certain guiding significance for early diagnosis and prevention of cognitive impairment.
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