The activation of microglia in the fetal brain of the offspring of a rat model of preeclampsia
Objective To observe the changes of microglia in prefrontal cortex,hippocampus and cerebellum of fetal brain of preeclampsia rats.Methods The abdominal aorta and bilateral uterine arteries were restrained to make a reduced uterine perfusion pressure(RUPP)model.The arterial blood pressure of the pregnant rat at 20.5 days was measured,and the total urine protein and pathological changes in kidneys were examined,immunofluorescence staining and Western blot were used to detect the expression of ionized calcium binding adaptor molecule-1(Iba1)in microglia in the prefrontal cortex,hippocampus and cerebellum.Results The arterial blood pressure test results showed that the arterial blood pressure of the RUPP group was significantly higher than that of the control group.The results of HE staining showed that compared with the control group,the volume of glomerular cells in the RUPP group was increased,and the number of cells in the glomerulus was increased.Western blot results showed that the protein expression of Iba1 in the prefrontal cortex of RUPP group embryos was increased compared with those of control embryos,but those in the hippocampus and cerebellum showed no difference.The results of immunofluorescence staining showed that the number of Iba1 positive cells in the prefrontal cortex of RUPP group embryos was increased compared with those of control embryos,and those in hippocampus and cerebellum showed no difference.Conclusion The proliferation of microglial cells in the prefrontal cortex of the fetal brain caursed by preeclampsia may be part of the reason for the abnormal neurodevelopment of the offspring.
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