摘要
目的 探究LRP8在宫颈癌组织中的表达与临床病理特征及预后的相关性,并探讨LRP8对宫颈癌细胞增殖、迁移和侵袭的影响及机制.方法 免疫组化检测LRP8在宫颈癌及癌旁组织中表达情况;根据免疫组化结果将30例宫颈癌组织分为LRP8低表达组和LRP8高表达组,分析LRP8表达与宫颈癌患者年龄、病理学TNM分期、临床分期和组织学分级间相关性;通过GEPIA在线数据库分析LRP8表达与宫颈癌患者总生存期相关性;将LRP8敲减或过表达质粒转染宫颈癌细胞,Western blot检测细胞LRP8表达,CCK-8检测细胞增殖活力,Transwell检测细胞迁移和侵袭能力,Western blot检测细胞β-catenin表达.结果 LRP8在宫颈癌组织中表达上调.LRP8高表达与宫颈癌患者较高的病理学T分期、病理学N分期和临床分期及较低的总生存期相关,与患者年龄、病理学M分期和组织学分级无关.敲减LRP8降低宫颈癌细胞增殖、迁移和侵袭能力及β-catenin蛋白表达水平;反之,过表达LRP8增加宫颈癌细胞增殖、迁移和侵袭能力及β-catenin蛋白表达水平.结论 LRP8在宫颈癌组织中高表达,其高表达与宫颈癌患者较高的病理学T分期、病理学N分期和临床分期及较低的总生存期相关,其可能通过激活Wnt/β-catenin信号通路促进宫颈癌细胞增殖、迁移和侵袭.
Abstract
Objective To explore the correlation between LRP8 expression in cervical cancer tissues and clinicopathological characteristics and prognosis,and to explore the effect and mechanism of LRP8 on the proliferation,migration and invasion of cervical cancer cells.Methods The expression of LRP8 in cervical cancer and adjacent tissues was detected by immunohistochemistry.According to the results of immunohistochemistry,30 cases of cervical cancer tissues were divided into LRP8 low expression group and LRP8 high expression group,and the correlation between LRP8 expression and age,pathological TNM stage,clinical stage and histological grade of cervical cancer patients was analyzed.The correlation between LRP8 expression and overall survival of cervical cancer patients was analyzed by GEPIA online database.LRP8 knockdown or overexpression plasmids were transfected into cervical cancer cells,and the LRP8 expression was detected by Western blot,the cell proliferation activity was detected by CCK-8,the cell migration and invasion ability was detected by Transwell,and the β-catenin expression was detected by Western blot.Results The expression of LRP8 was up-regulated in cervical cancer tissues.The high expression of LRP8 was associated with higher pathological T stage,pathological N stage,clinical stage and lower overall survival of cervical cancer patients,but not related to age,pathological M stage and histological grade.Knockdown of LRP8 reduced the proliferation,migration and invasion ability of cervical cancer cells and the expression level of β-catenin protein;conversely,overexpression of LRP8 increased the proliferation,migration and invasion ability of cervical cancer cells and the expression level of β-catenin protein.Conclusion LRP8 is highly expressed in cervical cancer tissues,and its high expression is associated with higher pathological T stage,pathological N stage,clinical stage and lower overall survival of cervical cancer patients.LRP8 may promote the proliferation,migration and invasion of cervical cancer cells by activating Wnt/β-catenin signaling pathway.
基金项目
沈阳医学院硕士研究生科技创新基金(Y20220527)
NETL
NSTL
万方数据