Effects of KRAS on PI3K/Akt signaling pathway and immune function in rats with lung cancer
Objective To analyze the effects of K-RAS oncogene(KRAS)on the PI3K/Akt signaling pathway and immune function in lung cancer rats.Methods 30 Wistar rats were selected,10 as the control group,and establish a lung cancer rat model.Twenty rats with successful model production were divided into model group and KRAS silencing group,with 10 rats in each group.The general situation of rats in each group were observed.The transfection efficiency was verified by RT-PCR method.Venous blood of rats was collected for immune function testing.Pathological changes in lung tissue of rats were observed by HE staining.The levels of CD3+、CD4+、CD4+/CD8+in serum were detected by flow cytometry.The expressions of PI3K and Akt mRNA and protein in lung tissue were detected by RT-PCR and Western blot.Results The results of RT-PCR showed that the KRAS lentivirus vector was successfully transfected.Compared with the model group,inhibition of KRAS could improve the pathological condition of lung tissue in lung cancer rats,upregulate the levels of CD3+、CD4+、CD4+/CD8+in serum,and decrease the expressions of PI3K and Akt mRNA and protein in lung tissues of rats.Conclusion Silencing the expression of KRAS could enhance the immune function of lung cancer rats,and its mechanism might be related to the inhibition of the PI3K/Akt signaling pathway.
lung cancerK-RAS proto-oncogenephosphatidylinositol 3-kinaseprotein kinase B
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