KRAS对肺癌大鼠PI3K/Akt信号通路与免疫功能的影响
Effects of KRAS on PI3K/Akt signaling pathway and immune function in rats with lung cancer
荀欣 1朱艾 1李馨如1
作者信息
- 1. 中国人民解放军联勤保障部队第九二○医院肿瘤科,云南昆明 650118
- 折叠
摘要
目的 分析K-RAS原癌基因(KRAS)对肺癌大鼠PI3K/Akt信号通路与免疫功能的影响.方法 选取30只Wistar大鼠,10只作为对照组,另外20只建立肺癌大鼠模型,将建模成功的20只大鼠分为模型组与KRAS沉默组,每组10只,观察各组大鼠一般情况,RT-PCR方法验证转染效率,采集各组大鼠静脉血进行检测免疫功能,HE染色观察各组大鼠肺组织病理学改变,流式细胞仪检测大鼠血清CD3+、CD4+、CD4+/CD8+表达,RT-PCR方法与Western blot方法检测大鼠肺组织中PI3K、Akt mRNA与蛋白的表达.结果 RT-PCR结果显示,KRAS慢病毒载体转染成功.与模型组相比,抑制KRAS表达可改善肺癌大鼠肺组织病理情况,上调大鼠血清CD3+、CD4+、CD4+/CD8+表达水平,降低肺组织PI3K与AktmRNA与蛋白表达水平.结论 沉默KRAS表达,可提高肺癌大鼠免疫功能,其机制可能与抑制PI3K/Akt信号通路有关.
Abstract
Objective To analyze the effects of K-RAS oncogene(KRAS)on the PI3K/Akt signaling pathway and immune function in lung cancer rats.Methods 30 Wistar rats were selected,10 as the control group,and establish a lung cancer rat model.Twenty rats with successful model production were divided into model group and KRAS silencing group,with 10 rats in each group.The general situation of rats in each group were observed.The transfection efficiency was verified by RT-PCR method.Venous blood of rats was collected for immune function testing.Pathological changes in lung tissue of rats were observed by HE staining.The levels of CD3+、CD4+、CD4+/CD8+in serum were detected by flow cytometry.The expressions of PI3K and Akt mRNA and protein in lung tissue were detected by RT-PCR and Western blot.Results The results of RT-PCR showed that the KRAS lentivirus vector was successfully transfected.Compared with the model group,inhibition of KRAS could improve the pathological condition of lung tissue in lung cancer rats,upregulate the levels of CD3+、CD4+、CD4+/CD8+in serum,and decrease the expressions of PI3K and Akt mRNA and protein in lung tissues of rats.Conclusion Silencing the expression of KRAS could enhance the immune function of lung cancer rats,and its mechanism might be related to the inhibition of the PI3K/Akt signaling pathway.
关键词
肺癌/K-RAS原癌基因/磷脂酰肌醇3-激酶/蛋白激酶BKey words
lung cancer/K-RAS proto-oncogene/phosphatidylinositol 3-kinase/protein kinase B引用本文复制引用
基金项目
联勤保障部队第九二○医院院内科技计划项目(2019YGC07)
出版年
2024
NETL
NSTL
万方数据