Propofol alleviates sepsis induced acute lung injury by activating the Nrf2/HO-1 signaling pathway
Objective To investigate whether propofol alleviates sepsis-induced acute lung injury by regulating the Nrf2/HO-1 signaling pathway.Methods Fifty healthy male C57BL/6 mice were randomly divided into control group(n=10),model group(n=20)and propofol group(n=20).Sepsis-induced acute lung injury was established by cecal ligation and puncture(CLP).The degree of pulmonary edema was evaluated by the ratio of lung wet weight to dry weight;the pathological changes of lung tissue were evaluated by HE staining;the levels of IL-1β,IL-6 and TNF-α in serum and the levels of MPO,MDA,SOD and GSH-Px in lung tissue were detected by ELISA;The protein expressions of NF-κB,Nrf2 and HO-1 were detected by Western blot.Result Propofol alleviated the pulmonary edema and pathological changes of lung tissue in mice induced by CLP,decreased the levels of serum inflammatory cytokines IL-1β,IL-6 and TNF-α(P＜0.05),decreased the expression levels of MPO and MDA in lung tissue,and increased the levels of SOD and GSH-Px(P＜0.05).The expression of Nrf2 and HO-1 in lung tissue were up-regulated,while the expression of NF-κB was down-regulated(P＜0.05).Conclusion Propofol alleviates sepsis induced acute lung injury by upregulating the Nrf2/HO-1 signaling pathway.
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