The influence of interleukin-34 on high glucose-induced epithelial-to-mesenchymal transition in rat kidney tubular epithelial cells and underlying mechanism
Objective To investigate the effect of interleukin-34(IL-34)on high glucose(HG)-induced epithelial-to-mesenchymal transition(EMT)in rat kidney tubular epithelial cell(NRK-52E cells)and its possible mechanism.Methods Renal tubular epithelial cells were cultured routinely and divided into normal control group,HG group(treated with 30 mMHG for 48 h),siRNA IL-34 transfection group and HG+siRNA IL-34 transfection group.The expressions of IL-34 protein was detected by enzyme-linked immunosorbent assay(ELISA).Phenotypic changes were observed by phase contrast microscope.The expressions of α-SMA,E-cadherin(E-ca),NF-κB,and p-NF-κB were detected by Western blot or immunofluorescence staining.Results After HG treatment,the protein expressions of IL-34 and α-SMA in NRK-52E cells were significantly increased,while the protein expression of E-ca was decreased,while siRNA IL-34 decreased the high expression of α-SMA and increased the protein expression of E-ca in renal tubular epithelial cells;Meanwhile,p-NF-κB protein expression was up-regulated in HG group,while siRNA IL-34 could down-regulate p-NF-κB protein expression.Conclusion IL-34 promotes EMT in renal tubular epithelial cells in HG,and the mechanism may be related to the activation of NF-κB signaling pathway.
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