FSTL1 inhibits proliferation and metastasis of lung cancer cells by inhibiting NF-κB/SPP1
Objective To elucidate the effects of FSTL1 on the proliferation and migration of human lung adenocarcinoma cells.Methods The experiment was divided into control group,10 ng/mL and 30 ng/mL recombinant FSTL1 group.Different concentrations of FSTL1 were used to treat CL1-5 cells.Cell proliferation was detected by CCK-8,and cell migration was detected by transwell test.Moreover,the expressions of MMP2,MMP9 and NF-κB/SPP1 were detected by western blot.Results FSTL1 significantly inhibited the proliferation of CL1-5 cells,and the higher the concentration,the stronger the inhibition.FSTL1also significantly decreased the migration of CL1-5 cells.After FSTL1 treatment,the expressions of MMP2 and MMP9 were significantly decreased in CL1-5 cells.Moreover,FSTL1 significantly decreased the expressions of NF-κB and SPP1.Conclusion FSTL1 can inhibit the proliferation and migration of CL1-5 cells,and its mechanism is related to the NF-κB/SPP1 signaling pathway.
human lung adenocarcinoma cellsFSTL1NF-κB/SPP1 pathway
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