FSTL1通过抑制NF-κB/SPP1抑制肺癌细胞的增殖和转移
FSTL1 inhibits proliferation and metastasis of lung cancer cells by inhibiting NF-κB/SPP1
宁欣 1佟昌慈 2郭天聪 1边明艳 1赵媛 1张佳莹1
作者信息
- 1. 辽宁省金秋医院呼吸科,辽宁沈阳 110016
- 2. 沈阳医学院树入国际学院,辽宁沈阳 110148
- 折叠
摘要
目的 探讨上调FSTL1对人肺腺癌细胞增殖、迁移的影响及可能分子机制.方法 将肺腺癌细胞株CL1-5分为对照组、10 ng/mL和30 ng/mL重组FSTL1组.CCK-8实验检测各组细胞的增殖能力;Transwell实验检测各组细胞的迁移能力;Western blot检测各组细胞的MMP2、MMP9和NF-κB/SPP1信号通路蛋白的表达.结果 FSTL1显著抑制CL1-5细胞增殖,且浓度越高对CL1-5细胞的增殖抑制作用越强;Transwell实验显示FSTL1可以明显抑制CL1-5细胞的迁移;FSTL1上调后,CL1-5细胞MMP2和MMP9的表达降低,NF-κB/SPP1信号通路被抑制.结论 FSTL1可能通过抑制NF-κB/SPP1信号通路抑制肺癌细胞的增殖和转移.
Abstract
Objective To elucidate the effects of FSTL1 on the proliferation and migration of human lung adenocarcinoma cells.Methods The experiment was divided into control group,10 ng/mL and 30 ng/mL recombinant FSTL1 group.Different concentrations of FSTL1 were used to treat CL1-5 cells.Cell proliferation was detected by CCK-8,and cell migration was detected by transwell test.Moreover,the expressions of MMP2,MMP9 and NF-κB/SPP1 were detected by western blot.Results FSTL1 significantly inhibited the proliferation of CL1-5 cells,and the higher the concentration,the stronger the inhibition.FSTL1also significantly decreased the migration of CL1-5 cells.After FSTL1 treatment,the expressions of MMP2 and MMP9 were significantly decreased in CL1-5 cells.Moreover,FSTL1 significantly decreased the expressions of NF-κB and SPP1.Conclusion FSTL1 can inhibit the proliferation and migration of CL1-5 cells,and its mechanism is related to the NF-κB/SPP1 signaling pathway.
关键词
人肺腺癌细胞/FSTL1/NF-κB/SPP1信号通路Key words
human lung adenocarcinoma cells/FSTL1/NF-κB/SPP1 pathway引用本文复制引用
基金项目
辽宁省自然科学基金(20170540951)
出版年
2024
NETL
NSTL
万方数据