小分子化合物G-749抑制胃癌细胞增殖和迁移
Small molecule compound G-749 suppresses the proliferation and migration of gastric cancer cells
蔺炜然 1张健 2李丰2
作者信息
- 1. 北部战区总医院检验科,辽宁沈阳 110016;中国医科大学生命科学学院分子细胞生物学教研室教育部医学细胞生物学重点实验室,辽宁沈阳 110122
- 2. 中国医科大学生命科学学院分子细胞生物学教研室教育部医学细胞生物学重点实验室,辽宁沈阳 110122
- 折叠
摘要
目的 研究小分子化合物G-749对人胃癌细胞MGC803和SGC7901的增殖和迁移的影响.方法 分别用不同浓度的G-749处理MGC803和SGC7901两种人胃癌细胞,CCK8实验和克隆形成实验检测G-749对胃癌细胞增殖的影响,流式细胞术检测化合物对细胞周期的影响,划痕实验检测G-749对胃癌细胞迁移的影响.结果 化合物G-749能够抑制人胃癌细胞MGC803和SGC7901的增殖并存在剂量依赖关系,抑制细胞G0/G1期向S期的转变,并能明显抑制胃癌细胞的迁移.结论 化合物G-749对胃癌细胞有抑制作用,有望应用于胃癌细胞的治疗.
Abstract
Objective To investigate the effects of small molecule compound G-749 on the proliferation and migration of human gastric cancer cells MGC803 and SGC7901.Methods MGC803 and SGC7901 human gastric cancer cells were treated with different concentrations of G-749.CCK8 assay and colony formation assay were used to detect the effect of G-749 on the proliferation of gastric cancer cells.Flow cytometry was used to check the effect of G-749 on the cell cycle.The scratch test was used to identity the effect of G-749 on the migration of gastric cancer cells.Results The compound G-749 inhibited the proliferation of human gastric cancer cells MGC803 and SGC7901 in a dose-dependent manner,and inhibited the transition of cells from G0/G1 phase to S phase,and suppressed the migration of gastric cancer cells.Conclusion Compound G-749 has inhibitory effect on gastric cancer cells and is expected to be used in the treatment of gastric cancer cells.
关键词
胃癌/G-749/增殖/细胞周期Key words
gastric cancer/G-749/proliferation/cell cycle引用本文复制引用
出版年
2024
NETL
NSTL
万方数据