BMP2复合壳聚糖水凝胶对大鼠胫骨骨折愈合的影响及可能机制
BMP2 combined with chitosan hydrogel mediates VEGFA/ERK1/2 signaling pathway to regulate angiogenesis and promote tibial fracture healing in rats
姚金池 1韩忠孝 1王雪 1吴腾飞 2王明月1
作者信息
- 1. 武警辽宁省总队医院外二科,辽宁沈阳 110034
- 2. 中国医科大学实验动物部,辽宁沈阳 110122
- 折叠
摘要
目的 探究BMP2复合壳聚糖水凝胶对大鼠胫骨骨折愈合的影响及可能机制.方法 将30只SD大鼠分为假手术组、模型组和BMP2复合壳聚糖水凝胶组,每组10只.HE染色检测大鼠骨折处病理形态改变;ELISA检测大鼠血清ALP水平;Western blot检测大鼠骨组织Ang-1、CD31、VEGFA、VEGFR2和p-ERK1/2蛋白表达水平.结果 与假手术组相比,胫骨骨折大鼠骨折愈合延迟,大鼠血清ALP水平及骨组织Ang-1、CD31、VEGFA、VEGFR2和p-ERK1/2蛋白表达水平降低;注射BMP2复合壳聚糖水凝胶促进胫骨骨折大鼠骨折愈合,增加大鼠血清ALP水平及骨组织Ang-1、CD31、VEGFA、VEGFR2和p-ERK1/2蛋白表达水平.结论 BMP2复合壳聚糖水凝胶诱导血管生成促进胫骨骨折大鼠骨折愈合,其机制可能与其激活VEGFA/ERK1/2信号通路有关.
Abstract
Objective To explore the effect of BMP2 combined with chitosan hydrogel on tibial fracture healing in rats and its possible mechanism.Methods Thirty SD rats were divided into sham operation group,model group and BMP2 combined with chitosan hydrogel group,with 10 rats in each group.HE staining was used to detect the pathological changes at the fracture site.The serum ALP levels were detected by ELISA.The expression levels of Ang-1,CD31,VEGFA,VEGFR2 and p-ERK1/2 in rat bone tissue were detected by Western blot.Results Fracture healing was delayed in tibial fracture rats,and the serum ALP level and the expression levels of Ang-1,CD31,VEGFA,VEGFR2 and p-ERK1/2 in bone tissue of rats were lower than those in sham operation group.The injection of BMP2 combined with chitosan hydrogel promoted the fracture healing of tibial fracture rats,increased the serum ALP level and the expression levels of Ang-1,CD31,VEGFA,VEGFR2 and p-ERK1/2 protein in bone tissue of rats.Conclusion BMP2 combined with chitosan hydrogel induces angiogenesis and promotes fracture healing in tibial fracture rats,which may be related to its activation of VEGFA/ERK1/2 signaling pathway.
关键词
壳聚糖/水凝胶/BMP2/骨折愈合/血管生成/VEGFA/ERK1/2信号通路Key words
chitosan/hydrogel/BMP2/fracture healing/angiogenesis/VEGFA/ERK1/2 signaling pathway引用本文复制引用
基金项目
辽宁省自然科学基金(2022-MS-188)
出版年
2024
NETL
NSTL
万方数据