LNC RNA Linc00152对宫颈癌细胞生物学行为及Fra-1/p53基因的影响
Effect of LNC RNA Linc00152 on biological behavior and Fra-1/p53 gene of cervical cancer cells
侯静 1吴樱 2李喜萍2
作者信息
- 1. 湖南省第二人民医院(湖南省脑科医院)妇产科,湖南长沙 410021
- 2. 长沙市第三医院妇产科,湖南长沙 410035
- 折叠
摘要
目的 探究LNC RNA Linc00152对宫颈癌细胞生物学行为及Fra-1/p53基因的影响.方法 RT-PCR方法检测人宫颈癌Hela细胞和人正常宫颈上皮End1/E6E7细胞中Linc00152表达;将Hela细胞分为正常组(control组)、转染组(si-00152)和转染对照组(si-NC),RT-PCR方法检测Linc00152的表达;MTT法检测细胞增殖能力;划痕实验检测细胞迁移能力;Transwell法检测细胞侵袭能力;Western blot检测细胞中Fra-1和p53蛋白表达.结果 Hela细胞中Linc00152的表达水平明显高于End1/E6E7细胞(P＜0.05).转染si-Linc00152后,Hela细胞Linc00152表达明显降低,增殖、迁移与侵袭能力明显降低(P＜0.05),p53表达明显升高,Fra-1表达明显降低(P＜0.05).结论 沉默LncRNA Linc00152表达能抑制Hela细胞的增殖、迁移和侵袭.
Abstract
Objective To explore the effect of LNC RNA Linc00152 on the biological behavior of cervical cancer cells and the Fra-1/p53 gene.Methods The expression of Linc00152 in human cervical cancer Hela cells and human normal cervical epithelial End1/E6E7 cells was detected by RT-PCR.Hela cells were divided into normal group(control group),transfection group(si-00152)and transfection control group(si-NC).The expression of Linc00152 was detected by RT-PCR.Proliferation of Hela cells was detected by MTT assay.Migration ability of Hela cells was detected by scratch test.Invasion ability of Hela cells was detected by Transwell method.The expressions of Fra-1 and p53 were detected by Western blot.Results The expression level of Linc00152 in Hela cells was significantly higher than that in End1/E6E7 cells(P＜0.05).After transfection of si-Linc00152,The expression of Linc00152 was decreased significantly,proliferation,migration and invasion of Hela cells were significantly decreased(P＜0.05),the expression of p53 was significantly increased,the expression of Fra-1 was significantly decreased(P＜0.05).Conclusion Silencing the expression of LncRNA Linc00152 inhibited the proliferation,migration and invasion of Hela cells.
关键词
LNC/RNA/Linc00152/宫颈癌/生物学行为/Fra-1/p53基因Key words
LNC RNA Linc00152/cervical cancer/biological behavior/Fra-1/p53 gene引用本文复制引用
基金项目
湖南省科技创新计划项目(2018JJ3782)
出版年
2024
NETL
NSTL
万方数据