The role of GPR124 in blood-brain barrier dysfunction after cerebral ischemia-reperfusion in rats and its possible mechanism
Objective To investigate the effect of GPR124 on blood-brain barrier dysfunction in rats with cerebral ischemia-reperfusion(I/R)and its possible mechanism.Methods Eighty SD rats were randomly divided into sham operation group,model group,GPR124 group and GPR124+FH535 group.The neurological function of rats in each group was evaluated by neurological function score;the brain edema and cerebral infarction area were calculated to evaluate the degree of brain injury in each group;the apoptosis of neurons and the structural changes of hippocampal tissues were analyzed by Nissl staining and H&E staining;the permeability of blood-brain barrier was analyzed by Evans blue staining.The effect of GRP124 on Wnt/β-catenin signaling pathway was analyzed by Western blot.Results Compared with the model group,after injection of recombinant protein GRP124,the neurological function defect score,brain edema,infarct area,neuron injury,blood-brain barrier permeability and the expression of Wnt/β-catenin signaling pathway-related proteins were significantly decreased in rats with cerebral I/R injury.After combined injection of GPR124+FH535,the above effects of GPR124 were weakened.Conclusion GPR124 can improve blood-brain barrier dysfunction in rats with cerebral I/R,and this effect may be related to the activation of Wnt/β-catenin signaling pathway by GPR124.
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