NCAPH在胶质瘤中的表达、预后及肿瘤恶性进展的关联研究
Correlation study of the expression,prognosis and malignant progression of NCAPH in glioma
郭兆刚 1岳博 1霍云龙 2邓超1
作者信息
- 1. 武警辽宁省总队医院神经外科,辽宁沈阳 110034
- 2. 中国医科大学附属盛京医院病理科,辽宁沈阳 110004
- 折叠
摘要
目的 研究非SMC粘合蛋白Ⅰ亚单位H(NCAPH)在胶质瘤的表达、预后以及参与的生物学功能.方法 下载并使用TCGA和GTEx数据库的表达谱数据分析NCAPH的表达水平;KM生存分析和COX回归分析NCAPH表达和患者预后的关系;GSEA分析NCAPH可能参与的生物学功能;ssGSEA分析NCAPH表达和免疫微环境的关系;RT-qPCR检测临床标本中NCAPH的表达水平.结果 胶质瘤中NCAPH的表达水平升高,且与患者预后和肿瘤的恶性进展有关.NCAPH的表达水平随着肿瘤的WHO分级的升高而升高,并且在IDH-1野生型患者中表达高于突变型.NCAPH可能参与肿瘤的增殖、迁移、促瘤炎症等生物功能.NCAPH与肿瘤微环境中的Th2细胞和巨噬细胞呈正相关,与其他细胞相关性则不明显.结论 NCAPH在胶质瘤中表达升高,可作为胶质瘤患者的预后标志物及治疗靶点.
Abstract
Objective To investigate the expression,prognosis and biological function of non-SMC adhesion protein Ⅰ subunit H(NCAPH)in glioma.Methods The expression profiles of NCAPH were downloaded and analyzed by TCGA and GTEx databases;the relationship between NCAPH expression and patient prognosis was analyzed by KM survival analysis and COX regression;the biological functions that NCAPH may be involved in were analyzed by GSEA;the relationship between NCAPH expression and immune microenvironment was analyzed by ssGSEA;and the expression level of NCAPH in clinical specimens was detected by RT-qPCR.Results The expression level of NCAPH in glioma was increased,and was related to the prognosis of patients and the malignant progression of tumors.The expression level of NCAPH increased with the increase of WHO grade of tumors,and the expression level of NCAPH in IDH-1 wild-type patients was higher than that in mutant patients.NCAPH may be involved in biological functions such as tumor proliferation,migration,tumor-promoting inflammation.NCAPH was positively correlated with Th2 cells and macrophages in tumor microenvironment,but not with other cells.Conclusion The expression level of NCAPH in glioma was increased,and NCAPH can be used as a prognostic marker and therapeutic target for glioma patients.
关键词
胶质瘤/TCGA/NCPAH/生物信息学Key words
glioma/TCGA/NCAPH/bioinformatics引用本文复制引用
基金项目
辽宁省科学技术基金(2021JH2/10300065)
出版年
2024
NETL
NSTL
万方数据