Effect of pachymic acid on proliferation,invasion and apoptosis of cervical cancer cells
Objective To investigate the effects and its regulatory mechanism of pachymic acid on the proliferation,invasion and apoptosis of cervical cancer cells.Methods Human cervical cancer Hela cells were cultured in vitro and treated with 5.0,10.0 and 20.0 μmol/L pachymic acid,respectively,and untreated Hela cells served as the control group.The effect of different concentrations of pachymic acid on cell proliferation was detected by MTT assay and clonal formation assay.The effect of different concentrations of pachymic acid on cell invasion was detected by trans well assay.The effect of different concentrations of pachymic acid on cell apoptosis was detected by flow cytometry.The expressions of Wnt,Cyclin D1 and matrix metalloproteinase(MMP)-2 were detected by Western blot.Results Compared with the control group,5.0,10.0 and 20.0 μmol/L pachymic acid could significantly inhibit the proliferation and invasion ability of Hela cells,and induce the apoptosis of Hela cells.The expression levels of Wnt,Cyclin D1 and MMP-2 in Hela cells were inhibited by different concentrations of pachymic acid in a concentration-dependent manner.Conclusion Pachymic acid can inhibit the proliferation and invasion of human cervical cancer Hela cells and induce apoptosis.
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