Protective effect and its possible mechanism of salvianolic acid B on lung tissue of young mice with mycoplasma pneumonia
Objective To explore the protective effect and its possible mechanism of salvianolic acid B on lung tissue of young mice with mycoplasma pneumonia.Methods Sixty SPF grade BALB/c young mice were randomly divided into control group(Control group),mycoplasma pneumonia group(MP group)and salvianolic acid B group(Sal B group),with 20 mice in each group.The morphological changes of lung tissue were observed by HE staining.The apoptosis of lung tissue cells of young mice in each group was observed by TUNEL method.The levels of interleukin-1β,interleukin-6 and tumor necrosis factor-α(TNF-α)in serum of young mice were detected by ELISA.The expressions of hypoxia-inducible Fact-1(HIF-1)and cleaved caspase-3 in lung tissues of young mice in each group were detected by Western blot.Results Salvianolic acid B could reduce the inflammation in lung tissue of young mice with mycoplasma pneumonia,reduce the apoptosis of lung tissue cells,reduce the levels of IL-1β,TNF-α and IL-6 in serum,and reduce the expressions of HIF-1 and cleaved caspase-3 in lung tissue of young mice with mycoplasma pneumonia.Conclusion Salvianolic acid B can reduce lung tissue injury in young mice with mycoplasma pneumonia by inhibiting the expressions of HIF-1 and cleaved caspase-3.
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