熊果酸抑制自噬增强结肠癌对奥沙利铂的敏感性
Ursolic acid enhances the sensitivity of colorectal Cancer to oxaliplatin by regulating autophagy
国星奇 1宿濛2
作者信息
- 1. 辽宁省肿瘤医院 大连理工大学附属肿瘤医院结直肠外科,辽宁沈阳 110042
- 2. 辽宁省肿瘤医院 大连理工大学附属肿瘤医院消化内科,辽宁沈阳 110042
- 折叠
摘要
目的 探讨熊果酸对结肠癌奥沙利铂敏感性的影响及可能机制.方法 构建Oxa耐药的结肠癌HCT116细胞,将HCT116细胞分为对照组(NC组)和熊果酸组(UA组).CCK8检测各组细胞活性;双染法检测各组细胞凋亡情况;Western blot检测各组细胞中LC3、P62自噬蛋白表达.结果 与对照组比较,熊果酸组HCT116对Oxa的敏感性增加,细胞中LC3-Ⅱ蛋白表达降低,P62表达升高;熊果酸可以促进Oxa引起的结肠癌细胞凋亡,而增加ATG7表达可减弱该效果.结论 熊果酸通过调控自噬增强结肠癌HCT116细胞对奥沙利铂的敏感性.
Abstract
Objective To investigate the effect of ursolic acid on the sensitivity of colorectal cancer cells to oxaliplatin and its possible mechanism.Methods Oxa-resistant colon cancer HCT116 cells were constructed and divided into control group(NC group)and ursolic acid group(UA group).Cell activity in each group was detected by CCK8.The apoptosis of cells in each group was detected by double staining.The expressions of LC3 and P62 were detected by Western blot.Results Compared with the control group,the sensitivity of HCT116 cells in ursolic acid group to Oxa was increased,the expression of LC3-Ⅱ was decreased,and the expression of P62 was increased.Ursolic acid could promote the apoptosis of colon cancer cells induced by Oxa,but increasing the expression of ATG7 could reduce the effect.Conclusion Ursolic acid enhances the sensitivity of colon cancer HCT116 cells to oxaliplatin by regulating autophagy.
关键词
结肠癌/熊果酸/自噬/奥沙利铂/耐药Key words
colorectal cancer/ursolic acid/autophagy/oxaliplatin/drug resistance引用本文复制引用
基金项目
辽宁省自然科学基金(2021-MS-073)
出版年
2024
NETL
NSTL
万方数据