Salusin-β promotes the inflammatory response in type 2 diabetic dry eye by regulating the NF-κB pathway
Objective To investigate the role of Salusin-β and NF-κB pathway in the inflammation of type 2 diabetic dry eye.Methods Thirty healthy male Wistar rats were randomly divided into blank control group,negative control group and Salusin-β low expression group,10 in each group.The pathological changes of rat conjunctiva were observed by HE staining.The levels of inflammatory factors in tears were measured by enzyme-linked immunosorbent assay.The expressions of Salusin-β and NF-κB p65 protein in rat conjunctiva was analyzed by Western blot.Results Compared with the negative control group,the ocular surface lesions of the rats in the Salusin-β low expression group were significantly alleviated,the tear secretion was increased,the corneal thickening,inflammatory cell infiltration and matrix edema were improved,the inflammatory factors in tears were significantly reduced,and the levels of Salusin-β and NF-κB p-p65 in conjunctiva were significantly decreased.Conclusion Salusin-β might promote inflammation in type 2 diabetic dry eye disease by regulating the NF-κB pathway.
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