Salusin-β调控NF-κB通路促进2型糖尿病性干眼病的炎症反应
Salusin-β promotes the inflammatory response in type 2 diabetic dry eye by regulating the NF-κB pathway
王豪 1欧升聪 2张学东3
作者信息
- 1. 重庆大学附属涪陵医院眼科,重庆 408030
- 2. 重庆市长寿区中医院眼科,重庆 401220
- 3. 重庆医科大学附属第一医院眼科,重庆 400010
- 折叠
摘要
目的 探究Salusin-β与NF-κB通路在2型糖尿病性干眼病炎症反应中的作用.方法 将30只健康雄性Wistar大鼠随机分为空白对照组、阴性对照组、Salusin-β低表达组,每组10只.HE染色观察大鼠眼结膜的病理结构变化;酶联免疫吸附法测定泪液炎症因子的水平;蛋白免疫印迹实验分析大鼠结膜组织Salusin-β和NF-κB p65蛋白的表达.结果 与阴性对照组比较,低表达组的大鼠眼表病变情况显著减轻、泪液分泌增加、角膜增厚、炎症细胞浸润和基质水肿的情况均得到改善、泪液中炎症因子显著降低、眼结膜Salusin-β和NF-κB p-p65蛋白表达水平显著降低.结论 Salusin-β可能通过调控NF-κB通路促进2型糖尿病性干眼病的炎症反应.
Abstract
Objective To investigate the role of Salusin-β and NF-κB pathway in the inflammation of type 2 diabetic dry eye.Methods Thirty healthy male Wistar rats were randomly divided into blank control group,negative control group and Salusin-β low expression group,10 in each group.The pathological changes of rat conjunctiva were observed by HE staining.The levels of inflammatory factors in tears were measured by enzyme-linked immunosorbent assay.The expressions of Salusin-β and NF-κB p65 protein in rat conjunctiva was analyzed by Western blot.Results Compared with the negative control group,the ocular surface lesions of the rats in the Salusin-β low expression group were significantly alleviated,the tear secretion was increased,the corneal thickening,inflammatory cell infiltration and matrix edema were improved,the inflammatory factors in tears were significantly reduced,and the levels of Salusin-β and NF-κB p-p65 in conjunctiva were significantly decreased.Conclusion Salusin-β might promote inflammation in type 2 diabetic dry eye disease by regulating the NF-κB pathway.
关键词
Salusin-β/NF-κB/2型糖尿病/干眼Key words
Salusin-β/NF-κB/Type 2 diabetes mellitus/dry eye引用本文复制引用
出版年
2024
NETL
NSTL
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