Effect of miR-132-3p targeting SIRT1 on repair and regeneration after sciatic nerve injury
Objective To explore the effects of miR-132-3p targeting SIRT1 on Schwann cell proliferation,apoptosis,and expression of nerve regeneration related proteins.Methods The potential binding sites of miR-132-3p and SIRT1 mRNA were analyzed by bioinformatics,and the double luciferase reporter gene experiment was used to verify their binding.Primary culture of Schwann cells,RT-qPCR and Western blot were used to detect the effect of miR-132-3p mimetics or inhibitors transfected on the expression of SIRT1 in Schwann cells.The SIRT1 over-expression plasmid was transfected into Schwann cells with miR-132-3p over-expression,and the expressions of S1RT1,GAP-43 and MBP were detected by Western blot.Cell proliferation activity was detected by CCK-8,and the apoptosis rate was detected by flow cytometry.Results miR-132-3p targeted SIRT1,miR-132-3p mimics decreased SIRT1 expression in Schwann cells,and miR-132-3p inhibitors increased SIRT1 expression in Schwann cells.Transfection of miR-132-3p mimics decreased Schwann cell proliferation activity and increased cell apoptosis rate,and decreased the expressions of GAP-43 and MBP.Overexpression of SIRT1 increased Schwann cell proliferation activity and decreased cell apoptosis rate,and increased the expression of GAP-43 and MBP in Schwann cells transfected with miR-132-3p mimics.Conclusion miR-132-3p inhibits Schwann cell proliferation and promotes apoptosis by inhibiting SIRT1 expression.
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