RBBP5在乳腺癌的表达及意义
Expression and significance of RBBP5 in breast cancer
芦洪波 1李佳 2郑振东2
作者信息
- 1. 锦州医科大学研究生院,辽宁锦州 121001;北部战区总医院肿瘤科,辽宁沈阳 110016
- 2. 北部战区总医院肿瘤科,辽宁沈阳 110016
- 折叠
摘要
目的 研究RBBP5在乳腺癌中的表达,探讨RBBP5的表达与乳腺癌临床分期、预后的关系及可能机制.方法 通过查找TCGA数据库中RBBP5基因在乳腺癌中的差异表达与临床分期、生存率的关系,利用基因富集分析(GSEA)软件对高表达RBBP5表型和低表达RBBP5表型进行功能富集分析;蛋白免疫印迹检测RBBP5表达情况;共聚焦荧光显微镜检测RBBP5的定位;流式细胞术分析RBBP5对细胞周期的影响.结果 TCGA数据库结果显示RBBP5基因在乳腺癌组织的表达明显高于癌旁组织(P＜0.05),其表达与临床分期和生存率相关(P＜0.05);免疫印迹证实RBBP5在多种乳腺癌细胞中均有表达;共聚焦荧光显微镜检测RBBP5主要定位在细胞核;流式细胞术结果显示RBBP5促进细胞周期进程.结论 RBBP5在乳腺癌中高表达,与预后呈负相关,可能参与乳腺癌细胞周期进程.
Abstract
Objective To study the expression of RBBP5 in breast cancer,and explore the relationship between the expression of RBBP5 and the clinical stage and prognosis of breast cancer and analyze the possible mechanism.Methods In this study,We first investigated the gene difference,survival rate,clinical stages of RBBP5 between BRCA and paracancerous tissues base in the TCGA database.Gene enrichment analysis(GSEA)software was used to analyze the functional enrichment of high and low expression RBBP5 phenotypes.The expression of RBBP5 was detected by Western blot,and the localization of RBBP5 was detected by confocal fluorescence microscopy.The effect of RBBP5 on cell cycle was analyzed by flow cytometry.Results The results of TCGA database showed that the expression of RBBP5 gene in breast cancer tissues was significantly higher than that in adjacent tissues(P＜0.05),and its expression was correlated with clinical stage and survival rate(P＜0.05).The result of western blot confirmed that RBBP5 was expressed in a variety of breast cancer cells.The localization of RBBP5 by confocal fluorescence microscopy was mainly in the nucleus.The result of flow cytometry showed that RBBP5 promoted cell cycle progression.Conclusion RBBP5 was highly expressed in breast cancer and negatively correlated with prognosis,which might be involved in the cell cycle progression of breast cancer.
关键词
RBBP5/乳腺癌/细胞周期Key words
RBBP5/breast cancer/cell cycle引用本文复制引用
出版年
2024
NETL
NSTL
万方数据