Expression and significance of RBBP5 in breast cancer
Objective To study the expression of RBBP5 in breast cancer,and explore the relationship between the expression of RBBP5 and the clinical stage and prognosis of breast cancer and analyze the possible mechanism.Methods In this study,We first investigated the gene difference,survival rate,clinical stages of RBBP5 between BRCA and paracancerous tissues base in the TCGA database.Gene enrichment analysis(GSEA)software was used to analyze the functional enrichment of high and low expression RBBP5 phenotypes.The expression of RBBP5 was detected by Western blot,and the localization of RBBP5 was detected by confocal fluorescence microscopy.The effect of RBBP5 on cell cycle was analyzed by flow cytometry.Results The results of TCGA database showed that the expression of RBBP5 gene in breast cancer tissues was significantly higher than that in adjacent tissues(P＜0.05),and its expression was correlated with clinical stage and survival rate(P＜0.05).The result of western blot confirmed that RBBP5 was expressed in a variety of breast cancer cells.The localization of RBBP5 by confocal fluorescence microscopy was mainly in the nucleus.The result of flow cytometry showed that RBBP5 promoted cell cycle progression.Conclusion RBBP5 was highly expressed in breast cancer and negatively correlated with prognosis,which might be involved in the cell cycle progression of breast cancer.
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