Effect of oleuropein on nerve cell apoptosis in rats with spinal cord injury and its possible mechanism
Objective To investigate the effects of oleuropein on apoptosis of nerve cells and JNK/p38 MAPK signaling pathway in rats with spinal cord injury.Methods Sixty 8-week-old female SD rats were randomly divided into sham group(Sham),SCI group(SCI)and oleuropein group(Oleuropein),with 20 rats in each group.On the 3rd day after spinal cord injury,BBB score was performed to evaluate the recovery of motor function.TUNEL test was used to detect apoptosis of nerve cells in rats with spinal cord injury.The expressions of phosphorylated JNK(p-JNK)and phosphorylated p38 MAPK(p-p38)in spinal cord tissues of rats were detected by immunofluorescence and Western blot.Results Compared with SCI group,the results of BBB score of rats in oleuropein group were significantly increased(P＜0.05),the apoptosis rate of nerve cells in spinal cord was significantly decreased(P＜0.05),and the expressions of p-JNK and p-p38 in spinal cord were significantly decreased(P＜0.05).Conclusion Oleuropein can reduce apoptosis of nerve cells by inhibiting JNK/p38 MAPK signaling pathway,thus alleviating spinal cord injury.
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