Objective To observe the effect of YAP on macrophage M1 polarization induced by LPS(Lipopolysaccharide).Methods PMA induces THP-1 and U937 cells to differentiate into macrophages(M0),then LPS continues to induce polarization to M1,RT-qPCR detected M1 marker,Rhodamine phalloidin was used to detect the changes of cytoskeleton.Western blot and RT-qPCR were used to detect the changes of Hippo pathway associated proteins and target genes.LPS induced M0 after reducing YAP expression,RT-qPCR was used to detect M1 markers changes.Results Western blot and RT-qPCR showed that the protein level of YAP and its target genes increased during the polarization of macrophages to M1;M1 markers were reduced after si YAP or treating cells with verteporfin compared to the control group.Conclusion YAP regulates LPS-induced macrophage polarization to Ml.
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