YAP调控LPS诱导的巨噬细胞M1型极化
YAP regulates LPS-induced M1 macrophage polarization
岳佩佩 1邹博 1张珂鑫 1朱晨 2吴彦菊 1王彪 1孟欣1
作者信息
- 1. 中国医科大学生命科学学院生物化学与分子生物学教研室,辽宁沈阳 110122
- 2. 中国医科大学附属第一医院神经外科,辽宁沈阳 110001
- 折叠
摘要
目的 探讨YAP对脂多糖(Lipopolysaccharide,LPS)诱导的巨噬细胞M1型极化的影响.方法 LPS诱导THP-1和U937细胞来源的M0型巨噬细胞向M1型极化.RT-qPCR法检测M1型巨噬细胞的标志物,罗丹明-鬼笔环肽检测细胞骨架,Western blot、RT-qPCR检测Hippo通路相关蛋白及其靶基因的表达.降低M0型巨噬细胞内YAP的表达,RT-qPCR检测LPS再诱导的M1型巨噬细胞的标志物的表达变化.结果 在M0型巨噬细胞向M1型极化过中,Hippo通路关键因子YAP的蛋白水平升高,下游靶基因的表达也升高;敲低YAP或使用维替泊芬抑制YAP表达后,LPS再诱导的M1型巨噬细胞标志物的表达明显降低.结论 YAP可以调控LPS诱导的巨噬细胞向M1极化.
Abstract
Objective To observe the effect of YAP on macrophage M1 polarization induced by LPS(Lipopolysaccharide).Methods PMA induces THP-1 and U937 cells to differentiate into macrophages(M0),then LPS continues to induce polarization to M1,RT-qPCR detected M1 marker,Rhodamine phalloidin was used to detect the changes of cytoskeleton.Western blot and RT-qPCR were used to detect the changes of Hippo pathway associated proteins and target genes.LPS induced M0 after reducing YAP expression,RT-qPCR was used to detect M1 markers changes.Results Western blot and RT-qPCR showed that the protein level of YAP and its target genes increased during the polarization of macrophages to M1;M1 markers were reduced after si YAP or treating cells with verteporfin compared to the control group.Conclusion YAP regulates LPS-induced macrophage polarization to Ml.
关键词
巨噬细胞/M1/极化/YAPKey words
macrophage/M1/polarization/YAP引用本文复制引用
基金项目
国家自然科学基金(81572831)
沈阳市科技计划(18-013-0-56)
出版年
2024
NETL
NSTL
万方数据