微小RNA-195-5p通过调控LRP6抑制滋养细胞增殖和转移
microRNA-195-5p inhibits the proliferation and metastasis of trophoblast cells by regulating LRP6
贺云云 1李润2
作者信息
- 1. 西安医学院第二附属医院产一科,陕西西安 710038
- 2. 西安医学院第二附属医院产二科,陕西西安 710038
- 折叠
摘要
目的 探讨微小RNA-195-5p对滋养细胞HTR-8/SVneo增殖、迁移和侵袭的影响及可能机制.方法 体外培养滋养细胞HTR-8/SVneo,将miR-195-5p模拟物转染进滋养细胞HTR-8/SVneo中,实时定量PCR方法检测各组细胞miR-195-5p和LRP6 mRNA的表达,CCK-8方法检测HTR-8/SVneo细胞增殖能力,Transwell实验检测HTR-8/SVneo细胞迁移和侵袭能力.结果 miR-195-5p模拟物可显著促进HTR-8/SVneo细胞中miR-195-5p的表达,并抑制LRP6 mRNA的表达(P＜0.05);双荧光素酶实验表明miR-195-5p可直接靶向LRP6;转染miR-195-5p模拟物后,HTR-8/SVneo细胞增殖、迁移和侵袭能力降低.结论 miR-195-5p可能通过抑制LRP6表达抑制HTR-8/SVneo细胞增殖、迁移和侵袭.
Abstract
Objective To investigate the effect and possible mechanism of miRNA-195-5p on proliferation,migration and invasion of trophoblast cells(HTR-8/SVneo).Methods Trophoblast cells(hTR-8/SVneo)were cultured in vitro,and miR-195-5p mimics were transfected into trophoblast cells(hTR-8/SVneo)to increase the expression of miR-195-5p.The expression of miR-195-5p and LRP6 mRNA were detected by real-time quantitative PCR in each group.The proliferation of HTR-8/SVneo cells were detected by CCK-8 assay,and the migration and invasion of HTR-8/SVneo cells were detected by Transwell assay.Results miR-195-5p mimics significantly promoted the expression of miR-195-5p and inhibited the expression of LRP6 mRNA in HTR-8/SVneo cells(P＜0.05).Dual luciferase assay showed that miR-195-5p could directly target LRP6.After transfection with miR-195-5p mimics,the proliferation,migration and invasion abilities of HTR-8/SVneo cells were decreased.Conclusion miR-195-5p may inhibit the proliferation,migration and invasion of HTR-8/SVneo cells by inhibiting the expression of LRP6.
关键词
微小RNA-195-5p/先兆子痫/增殖/侵袭Key words
miR-195-5p/preeclampsia/proliferation/invasion引用本文复制引用
基金项目
陕西省科技计划(2021)(2021SF-044)
出版年
2024
NETL
NSTL
万方数据