Ameliorating effect of jujuboside on insomnia model rats and its effect on cAMP/CREB/BDNF and PI3K/Akt pathways
Objective To explore the mechanism of jujuboside improving sleep in rats based on cAMP/CREB/BDNF and PI3K/Akt signaling pathways.Methods SD rats were randomly divided into blank control group,model group,jujuboside low dose group and high dose group.Sleep disorders were induced by p-chlorophenylalanine(PCPA).The general state of rats in each group was observed.Electroencephalogram(EEG)and electromyogram(EMG)signals were recorded by animal sleep bioanalytic system,and the total amount of Wake,non-rapid eye movement(NREM)and rapid eye movement(REM)sleep at night and day were analyzed.The contents of 5-hydroxytryptamine(5-HT),dopamine(DA)and cyclic adenosine phosphate(cAMP)were detected by ELISA.The expressions of cyclic adenosine phosphatide effer-binding protein(CREB),phosphorylated CREB(p-CREB),brain-derived neurotrophic factor(BDNF),phosphatidylinositol-3-hydroxykinase(PI3K),protein kinase B(Akt)and p-Akt protein in hypothalamus were detected by Western blot.Results After the intervention of jujuboside,the mental state of rats was significantly improved,the total amount of NREM sleep and REM sleep was significantly increased,the total amount of Wake was significantly decreased,the content of 5-HT and cAMP in hypothalamus was significantly increased,the content of DA was significantly decreased,and the expression levels of BDNF,p-CREB,PI3K and p-Akt were significantly increased.Conclusion Jujuboside has a certain effect on PCPA-induced sleep disorder in rats,and its mechanism of action may be related to the activation of cAMP/CREB/BDNF and PI3K/Akt signaling pathways.
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