Electroacupuncture improve neuroinflammation in mice with diabetic chronic inflammatory demyelinating polyradiculoneuropathy by mediating CXCR7/PI3K/Akt pathway
Objective To explore the mechanism of electroacupuncture in regulating macrophage polarization and improving neuroinflammation in mice with diabetic chronic inflammatory demyelinating polyradiculoneuropathy based on CXCR7/PI3K/Akt pathway.Methods Mice with a non-obese diabetic background knocked out of the co-stimulator protein CD86 were randomly divided into model group,electroacupuncture group,electroacupuncture+AMD3100 group and electroacupuncture+AMD3100+LY294002 group,and C57BL/6 mice of the same age were used as the control group.Neurological function scores were detected.The sciatic neuropathic injury was observed by HE staining.The levels of TNF-α,IFN-γ and IL-4 were measured by ELISA.The expressions of M1 macrophage marker protein(iNOS)and M2 macrophage marker protein(Arg-1)were detected by immunofluorescence staining.The expressions of CXCR7,PI3K,Akt,p-PI3K and p-Akt proteins were detected by Western blot.Results Compared with model group,the neurological function score was significantly decreased in electroacupuncture and electroacupuncture+AMD3100 group,the sciatic neuropathological injury was decreased,the levels of TNF-α and IFN-γ were significantly decreased,the level of IL-4 was significantly increased,the level of iNOS was significantly decreased,and the expression of Arg-1 was significantly increased.The expressions of CXCR7,p-PI3K and p-Akt were significantly increased.However,the above effects of electroacupuncture and AMD3100 on mice with mental disorders were weakened or inhibited by LY294002.Conclusion Electroacupuncture may promote the polarization of M1 macrophages to M2 by regulating the activity of CXCR7/PI3K/Akt pathway,thus improving the neuroinflammation of diabetic chronic inflammatory demyelinating polyradiculopathy mice.
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