Remimazolam in improving cerebral ischemia-reperfusion injury through miR-155-5p mediated DUSP14/TXNIP/NLRP3 pathway
Objective To investigate the mechanism of remimazolam in alleviating cerebral ischemia-reperfusion injury.Methods A total of 60 SD rats were randomly divided into sham group,model group,remimazolam group,miR-155-5p agomir NC group,miR-155-5p agomir group,remimazolam+miR-155-5p agomir group,with 10 rats in each group.A rat model of cerebral ischemia-reperfusion injury was established by wire plug method.The neurological deficit score of rats was detected.The pathological damage of brain tissue in rats were observed by HE staining.The apoptosis of nerve cells in the cerebral cortex was observed by TUNEL staining.The levels of IL-1β and IL-18 in brain tissues were detected by ELISA.The level of miR-155-5p in brain tissues was detected by qRT-PCR.The expressions of DUSP14,TXNIP and NLRP3 were measured by Western blot.Results Remimazolam improved neurological function,alleviated pathological damage of cerebral cortex,inhibited apoptosis of neuronal cells,reduced the levels of IL-1β and IL-18 in brain,reduced the level of miR-155-5p,increased the expression of DUSP14,and inhibited the expressions of TXNIP and NLRP3.However,the above effects of remimazolam on rats with cerebral ischemia-reperfusion injury were partially or fully reversed by miR-155-5p agomir.Conclusion Remimazolam could reduce cerebral ischemia-reperfusion injury by inhibiting the expression of miR-155-5p,mediating the activity of DUSP14/TXNIP/NLRP3 signaling pathway.
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