Protective effect of Jianganle on liver injury induced by isoniazid and rifampicin and its effect on Nrf-2/HO-1/NF-κB pathway in rats
Objective To explore the effect of Jianganle on nuclear factor E2 related factor(Nrf2)/heme oxygenase 1(HO-1)/nuclear factor kappa B(NF-κ B)pathway in liver tissue of rats with liver injury induced by isoniazid(INH)and rifampicin(RFP).Methods 60 SPF male SD rats were randomly divided into control group,model group,low dose Jianganle group and high dose Jianganle group.INH and RFP(150 mg/kg)were given by gavage to establish a liver injury model.Liver organ coefficient(LI)was calculated after administration.The liver morphology was observed by HE staining.The levels of oxidative stress and inflammation in serum were detected.Nuclear translocation of NF-κB in liver of rats was detected by immunofluorescence.The expressions of Nrf-2,HO-1 and NF-κB p65 in liver of rats were detected by Western blot.Results The levels of ALT,AST,ALP,activity of SOD and GSH-PX,content of GSH,levels of IL-1β,IL-6,TNF-α,and the expressions of Nrf2,HO-1 in liver and serum of liver injured rats were significantly decreased after treatment with low and high doses of Jianganle,and the fluorescence intensity of NF-κB nuclear translocation was significantly decreased.The level of MDA and the expression of NF-κB p65 protein in liver tissue were significantly increased(P＜0.05)in a dose-dependent manner.Conclusion Jianganle can reduce liver injury induced by isoniazid and rifampicin by regulating the Nrf2/HO-1/NF-κB signaling pathway.
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