BDNF-mTOR signaling mediated by sevoflurane to improve spatial learning and memory in hemorrhagic shock rats
Objective To explore the effect and mechanism of sevoflurane on spatial learning and memory ability of rats with hypothermia hemorrhagic shock.Methods 24 SPF male SD rats were randomly divided into sham group,HS group and sevoflurane treated hemorrhagic shock(Sev)group.The spatial learning and memory ability of rats was detected by morris water maze.The pathological changes of brain tissue were observed by HE staining.The expressions of ROS and BDNF in hippocampus were detected by immunofluorescence method.The structure and growth of neuronal dendrites in hippocampus of rats was observed by Golgi-Cox staining.The expressions of BDNF-mTOR signaling pathway related proteins in rat brain were detected by Western blot.Results The rats in hemorrhagic shock(HS)group showed abnormal behavior in morris water maze test,arrangement of brain cells was disordered,content of ROS in hippocampus was increased,neuronal dendrite structure was decreased,and the expressions of BDNF,p-TrkB and-mTOR were decreased significantly.After sevoflurane treatment,the performance and signal expression of rats in hypothermia hemorrhagic shock were improved.Conclusion Sevoflurane may improve the brain injury caused by hypothermia hemorrhagic shock and improve the spatial learning and memory ability of rats by regulating BDNF-mTOR signaling pathway.
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