摘要
目的 探讨养阴活胃合剂治疗慢性萎缩性胃炎(CAG)的作用机制.方法 将40只SD大鼠随机分为空白对照、模型组、养阴活胃合剂低、高剂量组,采用综合法[N-甲基-N,-硝基-N-亚硝基胍(MNNG)+雷尼替丁+不同浓度乙醇+饥饱失常法]复制大鼠CAG模型.观察大鼠一般状态、体重变化;ELISA方法检测大鼠血清胃蛋白酶原I(PGI)、胃蛋白酶原Ⅱ(PGⅡ)水平、白细胞介素-1β(IL-1β)、IL-6、IL-18水平;HE染色观察胃黏膜病理损伤;Western blot方法检测各组大鼠胃组织NLRP3、Caspase-1、GSDMD及GSDMD蛋白N端(GSDMD-N)蛋白表达.结果 与模型组比较,养阴活胃合剂低、高剂量可显著提高大鼠胃液酸度,改善胃液pH值,显著升高PGI和PGR水平,降低PGⅡ水平;明显减轻胃黏膜炎症;显著降低大鼠血清IL-1β、IL-6、IL-18水平;显著降低大鼠胃组织NL-RP3、Caspase-1、GSDMD及GSDMD-N蛋白表达水平.结论 养阴活胃合剂改善CAG大鼠胃黏膜炎症,可能是通过调控NLRP3/Caspase-1/GSDMD信号通路抑制细胞焦亡实现的.
Abstract
Objective To explore the mechanism of Yangyin Huowei mixture in treating chronic atrophic gastritis(CAG)based on NLRP3/Caspase-1/GSDMD signaling pathway.Methods Forty SD rats were randomly divided into control group,model group,low dose and high dose of Yangyin Huowei mixture group.The CAG model of rats was reproduced by comprehensive method(N-Methyl-N'-nitro-N-nitrosoguanidine+ranitidine+ethanol with different concentration+starvation and satiety disorder).The general state and body weight of rats were observed.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of pepsinogenⅠ(PGI),pepsinogen Ⅱ(PG Ⅱ)),interleukin-1β(IL-1β),IL-6,IL-18 levels in serum of rats.The pathological injury of gastric mucosa was observed by HE staining.The expressions of NLRP3,Caspase-1,GSDMD and GSDMD-N in gastric tissues were detected by Western blot.Results Compared with model group,low and high doses of Yangyin Huowei mixture could significantly increase the acidity of gastric juice,improve the pH value of gastric juice,significantly increase the levels of PGI and PGR,and decrease the level of PG Ⅱ,reduce inflammation in gastric mucosal,decrease the levels of IL-1β,IL-6 and IL-18 in serum,decrease the levels of NLRP3,Caspase-1,GSDMD and GSDMD-N in gastric tissue of rats.Conclusion Yangyin Huowei mixture improves gastric mucosal inflammation in CAG rats,which may be achieved by regulating NLRP3/Caspase-1/GSDMD signaling pathway to inhibit pyroptosis.
基金项目
新疆维吾尔自治区区域协同创新专项(上海合作组织科技伙伴计划及国际科技合作计划)(2022E01008)
新疆医科大学研究生创新创业项目(CXCY2023012)
NETL
NSTL
万方数据