CircSPECC1通过miR-409/SHP-2轴促进宫颈癌细胞增殖和迁移
CircSPECC1 promotes cervical cancer cell proliferation and migration through the miR-409/SHP-2 axis
朱赫 1孟斐2
作者信息
- 1. 湖州市妇幼保健院产科,浙江湖州 313002
- 2. 沈阳医学院附属中心医院产科,辽宁沈阳 110024
- 折叠
摘要
目的 探究circSPECC1对宫颈癌细胞增殖和迁移的影响及可能机制.方法 荧光原位杂交技术观察circSPECC1和miR-409-3p在宫颈癌细胞中共定位;双荧光素酶报告基因实验验证circSPECC1与miR-409-3p间结合;平板克隆形成实验检测过表达miR-409-3p对过表达circSPECC1的宫颈癌细胞克隆形成能力的影响;Tran-swell 实验检测过表达miR-409-3p对过表达circSPECC1的宫颈癌细胞迁移能力的影响.双荧光素酶报告基因实验验证SHP-2 mRNA与miR-409-3p间结合;Western blot实验检测过表达miR-409-3p或抑制miR-409-3p对宫颈癌细胞SHP-2蛋白表达的影响.结果 CircSPECC1和miR-409-3p在宫颈癌细胞中共定位于细胞浆.circSPECC1与miR-409-3p结合,且过表达miR-409-3p逆转了过表达circSPECC1对宫颈癌细胞克隆形成能力和迁移能力的促进作用.SHP-2 mRNA与miR-409-3p结合,抑制miR-409-3p上调宫颈癌细胞SHP-2蛋白表达,且过表达miR-409-3p降低宫颈癌细胞SHP-2蛋白表达.结论 CircSPECC1通过结合miR-409-3p促进宫颈癌细胞增殖和迁移,其机制可能与海绵化miR-409-3p上调SHP-2表达有关.
Abstract
Objective To explore the effect and mechanism of circSPECC1 on the proliferation and migration of cervical cancer cells.Methods The co-localization of circSPECC1 and miR-409-3p in cervical cancer cells was observed by fluorescence in situ hybridization.Dual luciferase reporter gene assay confirmed circSPECC1 binding to miR-409-3p.The effect of overexpression of miR-409-3p on the clonogenesis of cirCSPECC1-overexpressed cervical cancer cells was detected by plate cloning assay.The effect of overexpression of miR-409-3p on the migration ability of cirCSPECC1-overexpressed cervical cancer cells was detected by transwell assay.The binding between SHP-2 mRNA and miR-409-3p was verified by double luciferase reporter gene assay.The effects of overexpression or inhibition of miR-409-3p on the expression of SHP-2 protein in cervical cancer cells were detected by Western blot.Results CircSPECC1 and miR-409-3p was co-localized in the cytoplasm of cervical cancer cells.CircSPECC1 binded to miR-409-3p,and overexpression of miR-409-3p reversed the promoting effect of overexpression of circSPECC1 on the cloning and migration ability of cervical cancer cells.SHP-2 mRNA binded to miR-409-3p.Inhibition of miR-409-3p upregulated the expression of SHP-2 in cervical cancer cells and overexpression of miR-409-3p reduced the expression of SHP-2 in cervical cancer cells.Conclusion CircSPECC1 promotes cervical cancer cell proliferation and migration by binding to miR-409-3p,which may be related to the upregulation of SHP-2 expression by sponge miR-409-3p.
关键词
CircSPECC/1/宫颈癌/miR-409/SHP-2轴/增殖/迁移Key words
circSPECC1/cervical cancer/miR-409/SHP-2 axis/proliferation/migration引用本文复制引用
基金项目
辽宁省教育厅2021年度科学研究经费面上项目(LJKZ1159)
出版年
2024
NETL
NSTL
万方数据