Paeoniflorin improved sevoflurane-induced cognitive impairment in elderly rats by regulating the BDNF/TrkB signaling pathway
Objective To explore the effect and the mechanism of paeoniflorin on cognitive impairment induced by sevoflurane in elderly rats.Methods Thirty-six SD rats were randomly divided into control group(Control),sevoflurane group(Model)and treatment group(Paeoniflorin).The learning and memory ability of rats in each group were detected by water maze experiment,and the morphology of hippocampal neurons were observed by HE staining and Nith staining.The apoptosis of hippocampal nerve cells was detected by flow cytometry.Western blot analysis was performed to detect the expressions of Bax,Bcl-2,BDNF and TrkB in the hippocampus of rats in each group.Results Compared with sevoflurane group,paeoniflorin treatment could shorten the incubation period of rats and increase the times of crossing the platform(P＜0.05),alleviates the atrophy of rat neuron cells and the dissolution of Nisherian micromanifestations caused by sevoflurane treatment,decrease the apoptosis rate of rat hippocampal nerve cells,the expression of Bax,and up-regulate the expressions of Bcl-2,BDNF and TrkB(P＜0.05).Conclusion Paeoniflorin improves cognitive dysfunction in aged rats induced by sevoflurane by regulating BDNF/TrkB signaling pathway.
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