microRNA-222-3p inhibits the proliferation,migration and invasion of mantle cell lymphoma by targeting STAT5B
Objective To investigate the level of microRNA-222-3p(miR-222-3p)in mantle cell lymphoma and its effect on proliferation,migration and invasion of cells.Methods The expression of miR-222-3p in human mantle cell lymphoma cell line was detected by qRT-PCR.Proliferation of cells was detected by CCK-8 assay.Migration and invasion of cells were detected by Transwell assay.The expression of STAT5B was detected by Western blot assay.Dual luciferase reporter gene assay verified the binding relationship between miR-222-3p and STAT5B.Results The level of miR-222-3p in human mantle cell lymphoma cells(JVM-2 and Z138)was significantly lower than that of normal B lymphocytes IM-9.Overexpression of miR-222-3p significantly inhibited the proliferation,migration and invasion of human mantle cell lymphoma cells,and the expression level of STAT5B protein was inhibited.Dual luciferase assay showed that miR-222-3p could bind to STAT5B,and high expression of STAT5B could partially reverse the inhibitory effect of miR-222-3p mimics on proliferation,migration and invasion of human mantle cell lymphoma cells.Conclusion The level of miR-222-3p in human mantle cell lymphoma cells was significantly lower than that in normal B lymphocytes,and miR-222-3p could inhibit the proliferation,migration and invasion of human mantle cell lymphoma cells by regulating STAT5B.
NETL
NSTL
万方数据
