微小RNA-222-3p通过靶向STAT5B抑制套细胞淋巴瘤增殖,迁移和侵袭
microRNA-222-3p inhibits the proliferation,migration and invasion of mantle cell lymphoma by targeting STAT5B
和芳 1刘建琼 2高小丽 2俞娟 1马莎 1赵维庆 1张明星 1王娟 1陆智祥2
作者信息
- 1. 云南省第一人民医院风湿免疫科,云南昆明 650034
- 2. 云南省第一人民医院血液科,云南昆明 650034
- 折叠
摘要
目的 研究套细胞淋巴瘤中miR-222-3p的表达水平及对人套细胞淋巴瘤细胞增殖、迁移和侵袭的影响.方法 体外培养人套细胞淋巴瘤细胞系JVM-2和Z138,以及正常B淋巴细胞系IM-9.将miR-222-3p模拟物分别转染进JVM-2和Z138细胞系中构建miR-222-3p高表达细胞模型,qRT-PCR方法检测人套细胞淋巴瘤细胞系中miR-222-3p的表达;CCK-8实验检测细胞的增殖;Transwell实验检测细胞迁移和侵袭;Western blot实验检测STAT5B蛋白的表达;双荧光素酶报告基因实验验证miR-222-3p和STAT5B的结合关系.结果 miR-222-3p在人套细胞淋巴瘤细胞(JVM-2和Z138)中的表达水平显著低于正常B淋巴细胞IM-9.过表达miR-222-3p可显著抑制人套细胞淋巴瘤细胞的增殖、迁移和侵袭;抑制STAT5B蛋白的表达水平.双荧光素酶报告实验显示miR-222-3p可与STAT5B结合,高表达STAT5B可部分逆转miR-222-3p模拟物对人套细胞淋巴瘤细胞的增殖、迁移和侵袭的抑制作用.结论 miR-222-3p在人套细胞淋巴瘤细胞中表达水平显著低于正常B淋巴细胞,其可通过调控STAT5B抑制人套细胞淋巴瘤细胞增殖、迁移和侵袭.
Abstract
Objective To investigate the level of microRNA-222-3p(miR-222-3p)in mantle cell lymphoma and its effect on proliferation,migration and invasion of cells.Methods The expression of miR-222-3p in human mantle cell lymphoma cell line was detected by qRT-PCR.Proliferation of cells was detected by CCK-8 assay.Migration and invasion of cells were detected by Transwell assay.The expression of STAT5B was detected by Western blot assay.Dual luciferase reporter gene assay verified the binding relationship between miR-222-3p and STAT5B.Results The level of miR-222-3p in human mantle cell lymphoma cells(JVM-2 and Z138)was significantly lower than that of normal B lymphocytes IM-9.Overexpression of miR-222-3p significantly inhibited the proliferation,migration and invasion of human mantle cell lymphoma cells,and the expression level of STAT5B protein was inhibited.Dual luciferase assay showed that miR-222-3p could bind to STAT5B,and high expression of STAT5B could partially reverse the inhibitory effect of miR-222-3p mimics on proliferation,migration and invasion of human mantle cell lymphoma cells.Conclusion The level of miR-222-3p in human mantle cell lymphoma cells was significantly lower than that in normal B lymphocytes,and miR-222-3p could inhibit the proliferation,migration and invasion of human mantle cell lymphoma cells by regulating STAT5B.
关键词
套细胞淋巴瘤/miR-222-3p/STAT5B/增殖/迁移/侵袭Key words
mantle cell lymphoma/miR-222-3p/STAT5B/proliferation/migration/invasion引用本文复制引用
基金项目
云南省应用基础研究专项-昆医联合面上项目(202001AY070001-121)
云南省第一人民医院血液疾病临床医学中心开放项目(2022LCZXKF-XY05)
出版年
2024
NETL
NSTL
万方数据