The protective effect of sodium houttuyfonate on kidney stones via VEGF signaling pathway in rats
Objective To investigate the protective effect of houttuydin sodium(SH)on kidney stones via VEGF signaling pathway in rats.Methods Fifty adult male SD rats were randomly divided averagely into control group,model group,low-dose SH group(60mg/kg),and middle-dose SH group(120 mg/kg),SH high-dose group(240 mg/kg).The control group was given 2 mL/d of normal saline,and the other groups were given an equal amount of 0.75％ethylene glycol+0.75％ammonium chloride solution to the stomach each day to establish a kidney stone model for 28 days.The body weight and kidney weight were determined to calculate the renal index.24 h urine volume,contents of oxalic acid(Ox)and citric acid(CA),Ca2+concentration in urine,and the contents of urea nitrogen(BUN)and creatinine(Cr)and Ca2+in serum were detected.HE and Pizzolato's staining were used to observe the formation of calcium oxalate(CaOx)crystals in the kidney;immunohistochemical methods were used to observe blood vessels in the renal tissue of rats.Results Compared with the control group,the body weight of the rats in the model group was significantly reduced,and the renal index was significantly increased.The levels of Ox,Ca2+in urine and BUN,Cr and Ca2+in serum were all significantly increased.24 h urine volume and CA content in urine were decreased significantly,CaOx crystal deposition was increased in kidney tissues,and VEGF expression was increased.Conclusion The protective effect of SH on kidney stones is related to the down-regulation of VEGF in kidney of SD rat.
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