The effect of sevoflurane anesthesia on cognitive function via inhibition of PI3K/Akt/eNOS in rats
Objective To investigate the effects of anesthesia with sevoflurane on hippocampal dendritic spines and their cognitive function in rats.Methods Forty-eight SD rats were randomly divided into normal control group,3％sevoflurane-6h group,3％sevoflurane-3h group,and 3％sevoflurane-1 h group,with 12 rats in each group.24 hours later,the water maze test was performed,and the escape latency,the number of crossing the platform,and the cruise time in the target quadrant were recorded.The expressions of PI3K/Akt/eNOS signaling pathway-related proteins in hippocampus were detected by Western blot.Na+-K+-ATPase activity was detected by kit,and dendritic spine status was detected by Golgi staining.Results The escape latency was increased,but the expression level of PI3K protein,and Akt and eNOS protein phosphorylation levels were significantly decreased,Na+-K+-ATPase activity reduced,and the density and length of hippocampal dendritic spines was significantly decreased in the sevoflurane-6h group than in the control group.Conclusion Excessive inhalation of sevoflurane leads to cognitive impairment,which is related to inhibiting the activation of related proteins in the PI3K/Akt/eNOS signaling pathway,inhibiting the expression of Na+-K+-ATPase activity and damaging dendritic cells in the hippocampus of rats.
sevofluraneeNOSNa+-K+-ATPasedendritic spinescognitive functionSD rat
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