Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia
Objective To observe the expression and localization of group Ⅰ metabotropic glutamate receptors(mGluR1/5)in rat superior cervical ganglion(SCG)and the effect of chronic intermittent hypoxia(CIH)on mGluR1/5 protein level.Methods Twelve male SD rats were randomly divided into control group(Ctrl)and CIH group(CIH),6 rats in each group.After 6 weeks of modeling,the effect of CIH on mGluR1/5 protein level was detected by Western blotting,the expression and distribution of mGluR1/5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining.Results mGluR1/5 was expressed in rat SCG.mGluR1 was distributed in neurons and small intensely fluorescent(SIF)cells,but not in satellite glial cells(SGCs),nerve fibers and blood vessels,whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons,but not in SGCs,SIF cells and blood vessels.CIH increased the protein levels of mGluR1/5(P＜0.01)in rat SCG.Conclusion Both mGluR1 and mGluR5 are expressed in the rat SCG,but their distribution are different,and the increased protein levels of both may be involved in CIH-induced hypertension.
Group Ⅰ metabotropic glutamate receptorSuperior cervical ganglionChronic intermittent hypoxiaHypertensionWestern blottingRat
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