首页|神经肌肉电刺激通过调控白细胞介素6/信号转导子及转录激活子3信号通路促进脊髓损伤后小鼠运动功能恢复

神经肌肉电刺激通过调控白细胞介素6/信号转导子及转录激活子3信号通路促进脊髓损伤后小鼠运动功能恢复

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目的 观察神经肌肉电刺激(NMES)对脊髓损伤后小鼠白细胞介素 6(IL-6)/STAT3 信号通路的影响，探讨其对运动功能恢复的机制。方法 选取SPF级小鼠 72 只随机分成假手术(sham)组、脊髓损伤组(SCI)和NMES组。利用BMS评分、斜坡实验与电生理(EMG)评估小鼠脊髓损伤后肢体运动恢复情况。Western blotting和Real-time PCR检测各组小鼠脊髓组织中相关炎症因子、IL-6/STAT3 信号通路与胶质纤维酸性蛋白(GFAP)和脑源性神经营养因子(BDNF)的表达。HE染色观察各组小鼠脊髓形态结构。结果 NMES组BMS评分和小鼠斜坡实验均高于SCI组(P＜0。05);NMES组小鼠运动诱发电位(MEP)最大振幅高于SCI组(P＜0。05);NMES组脊髓组织TNF-α、IL-12A与GFAP表达量均低于SCI组(P＜0。05)，TGF-β、IL-10 与BDNF表达量均高于SCI组(P＜0。05);与SCI组相比，NMES组小鼠脊髓空洞较少，脊髓形态修复较好;与SCI组相比，NMES组IL-6/STAT3 信号通路蛋白表达均低于SCI组(P＜0。05)。结论 神经肌肉电刺激通过抑制IL-6/STAT3 信号通路发挥抗炎作用，从而促进脊髓损伤后小鼠后肢运动功能的恢复。
Neuromuscular electrical stimulation promoting the recovery of motor function in mice after spinal cord injury by regulating interleukin-6/signal transducer and activator of transcription-3 signaling pathway
Objective To observe the effect of neuromuscular electrical stimulation(NMES)on interleukin-6(IL-6)/STAT3 signaling pathway in mice after spinal cord injury,and to explore the mechanism of its effect on motor function recovery.Methods Seventy-two SPF grade mice were randomly divided into sham operation group,spinal cord injury(SCI)group and NMES group.BMS score,inclined plane test and neuromuscular electrophysiology(EMG)were used to evaluate the recovery of spinal cord injury in mice.Western blotting and Real-time PCR were used to detect the expression of inflammatory factors,IL-6/STAT3,glial fibrillary acidic protein(GFAP)and brain-derived neurotrophic factor(BDNF)in spinal cord tissues of three groups of mice.HE staining was used to observe the pathological changes of spinal cord injury.Results BMS scores and the inclined plane test of mice in the NMES group were higher than those in SCI group(P＜0.05).The maximum amplitude of motor evoked potential in NMES group was higher than that in SCI group(P＜0.05).The expressions of TNF-α,IL-12A and GFAP in the spinal cord of NMES group were lower than that of SCI group(P＜0.05),while the expressions of TGF-β,IL-10 and BDNF were higher than that of SCI group(P＜0.05).The protein expressions of IL-6/STAT3 signaling pathway of NMES group were lower than that of SCI group(P＜0.05).Conclusion Neuromuscular electrical stimulation plays an anti-inflammatory role by inhibiting the IL-6/STAT3 signaling pathway,thereby promoting the recovery of hind limb motor function in mice after spinal cord injury.

Spinal cord injuryInflammationInterleukin-6/signal transducer and activator of transcription-3 signaling pathwayNeuromuscular electrical stimulationWestern blottingMouse

覃建锋、宋海旺、孙宝飞、吉杨丹、龙思方、杨丹

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黔南民族医学高等专科学校基础医学部,贵州 都匀 558003

贵州医科大学基础医学院人体解剖学教研室,贵州 贵安新区 561113

脊髓损伤 炎症反应 白细胞介素6/信号转导子及转录激活子3信号通路 神经肌肉电刺激 免疫印迹法 小鼠

贵州省科技厅项目贵州医科大学博士启动基金黔南民族医学高等专科学校科研项目黔南民族医学高等专科学校科研项目

黔科合基础-ZK[2021]一般472校博合J字[2020]009号Qnyz202001Qnyz202207

2024

10.16098/j.issn.0529-1356.2024.03.002

解剖学报
中国解剖学会

解剖学报

CSTPCD
影响因子：0.462
ISSN：0529-1356
年,卷(期)：2024.55(3)
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