Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men
Objective To explore the relationship between sperm DNA fragmentation index(DFI)and sperm quality,as well as in vitro fertilization-embryo transfer(IVF-ET)preganancy outcomes in middle-aged men.Methods A total of 180 semen specimens from males aged over 38 years who received comventional IVF-ET treatment were collected and divided into two groups(＜30%and≥30%)based on the DIF threshold.Main measures result including:normal semen parameters,hormone levels,DFI,fertilization rate,good quality embryo rate and pregnancy rate.Results DFI was closely related to male follicle-stimulating hormone(FSH),sperm motility,morphology,and the sperm vitality decreased with increasing DFI level(P＜0.05).When DFI≥30%,the rate of high-quality embryos decreased(P＜0.05),but there was no significant difference in the pregnancy rate(P＞0.05).Conclusion DFI can serve as an important indicator for routine analysis of semen in middle-aged men.DFI affects the rate of high-quality embryos,but it is not related to the clinical pregnancy outcome of assistant reproduction technology(ART).
DNA fragmentation indexSperm motilitySperm chromatin dispersionMorphological observationHuman
NETL
NSTL
万方数据
