中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响
Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men
王平 1王静 1陈先兵 1陈曦1
作者信息
- 1. 北京大学人民医院妇产科生殖医学中心,北京 100044
- 折叠
摘要
目的 探讨中年男性精子DNA碎片率(DFI)与精液质量和体外受精-胚胎移植妊娠结局的关系.方法 共收集 180 例接受常规体外受精-胚胎移植治疗且男性年龄＞38 岁的精液标本.根据DFI的阈值分成(＜30%和≥30%)两组.主要测量指标包括:常规精液参数、激素水平、DFI、受精率、优质胚胎率、临床妊娠率等.结果 通过比较分析发现,DFI与男性卵泡刺激素(FSH)、精子活力、精子形态密切相关,且精子活力随着DFI水平的升高而下降(P＜0.05);当DFI≥30%时,优质胚胎率下降(P＜0.05),但两组的临床妊娠率差异无显著性(P＞0.05).结论 DFI可以作为中年男性精液常规分析的重要参考指标,虽然DFI影响优质胚胎率,但与辅助生殖治疗的临床妊娠结局无关.
Abstract
Objective To explore the relationship between sperm DNA fragmentation index(DFI)and sperm quality,as well as in vitro fertilization-embryo transfer(IVF-ET)preganancy outcomes in middle-aged men.Methods A total of 180 semen specimens from males aged over 38 years who received comventional IVF-ET treatment were collected and divided into two groups(＜30%and≥30%)based on the DIF threshold.Main measures result including:normal semen parameters,hormone levels,DFI,fertilization rate,good quality embryo rate and pregnancy rate.Results DFI was closely related to male follicle-stimulating hormone(FSH),sperm motility,morphology,and the sperm vitality decreased with increasing DFI level(P＜0.05).When DFI≥30%,the rate of high-quality embryos decreased(P＜0.05),but there was no significant difference in the pregnancy rate(P＞0.05).Conclusion DFI can serve as an important indicator for routine analysis of semen in middle-aged men.DFI affects the rate of high-quality embryos,but it is not related to the clinical pregnancy outcome of assistant reproduction technology(ART).
关键词
精子DNA碎片率/精子活力/精子染色质扩散/形态学观察/人Key words
DNA fragmentation index/Sperm motility/Sperm chromatin dispersion/Morphological observation/Human引用本文复制引用
出版年
2024
NETL
NSTL
万方数据